@article{7a25551090c34473b9d35c62e16d91cf,
title = "Reverse Complement PCR: A novel one-step PCR system for typing highly degraded DNA for human identification",
abstract = "Reverse Complement PCR (RC-PCR) is an innovative, one-step PCR target enrichment technology adapted for the amplification of highly degraded (fragmented) DNA. It provides simultaneous amplification and tagging of a targeted sequence construct in a single, closed-tube assay. A human identification (HID) RC-PCR panel was designed targeting 27 identity single nucleotide polymorphisms (SNPs) generating targets only 50 base pairs in length. In a single reaction, the complete sequencing construct is produced which is essential for massively parallel sequencing (MPS) library preparation, thus reducing time and labor as well as minimizing the risk of sample carry-over or other forms of contamination. The RC-PCR system was evaluated and found to produce reliable and concordant variant calls. Also, the RC-PCR system demonstrated to have substantial sensitivity of detection with a majority of alleles detected at 60 pg of input DNA and robustness in tolerating known PCR inhibitors. The RC-PCR system may be an effective alternative to current forensic genetic methods in the analysis of highly degraded DNA.",
keywords = "Degraded DNA, Identity SNPs, RC-PCR, Reverse Complement PCR, SNPs",
author = "Kieser, {Rachel E.} and Bu{\'s}, {Magdalena M.} and King, {Jonathan L.} and {van der Vliet}, Walter and Joop Theelen and Bruce Budowle",
note = "Funding Information: The authors would like to thank Dixie Peters from the University of North Texas Center for Human Identification (UNTCHI) for her guidance and support in the identification and procurement of the challenged forensic casework samples for evaluation of the RC-PCR technology. This work was funded by Award No. 2016-DN-BX-0154, awarded by the National Institute of Justice (NIJ), Office of Justice Programs, U.S. Department of Justice. The opinions, findings, and conclusions expressed within this publication are those of the author(s) and do not necessarily reflect those of the U.S. Department of Justice. Funding Information: The authors would like to thank Dixie Peters from the University of North Texas Center for Human Identification (UNTCHI) for her guidance and support in the identification and procurement of the challenged forensic casework samples for evaluation of the RC-PCR technology. This work was funded by Award No. 2016-DN-BX-0154 , awarded by the National Institute of Justice (NIJ), Office of Justice Programs, U.S. Department of Justice . The opinions, findings, and conclusions expressed within this publication are those of the author(s) and do not necessarily reflect those of the U.S. Department of Justice. Appendix A Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",
year = "2020",
month = jan,
doi = "10.1016/j.fsigen.2019.102201",
language = "English",
volume = "44",
journal = "Forensic Science International: Genetics",
issn = "1872-4973",
publisher = "Elsevier Ireland Ltd",
}