Retinal angiogenesis suppression through small molecule activation of p53

Sai H. Chavala, Younghee Kim, Laura Tudisco, Valeria Cicatiello, Till Milde, Nagaraj Kerur, Nidia Claros, Susan Yanni, Victor H. Guaiquil, William W. Hauswirth, John S. Penn, Shahin Rafii, Sandro De Falco, Thomas C. Lee, Jayakrishna Ambati

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Neovascular age-related macular degeneration is a leading cause of irreversible vision loss in the Western world. Cytokine-targeted therapies (such as anti-vascular endothelial growth factor) are effective in treating pathologic ocular angiogenesis, but have not led to a durable effect and often require indefinite treatment. Here, we show that Nutlin-3, a small molecule antagonist of the E3 ubiquitin protein ligase MDM2, inhibited angiogenesis in several model systems. We found that a functional p53 pathway was essential for Nutlin-3-mediated retinal antiangiogenesis and disruption of the p53 transcriptional network abolished the antiangiogenic activity of Nutlin-3. Nutlin-3 did not inhibit established, mature blood vessels in the adult mouse retina, suggesting that only proliferating retinal vessels are sensitive to Nutlin-3. Furthermore, Nutlin-3 inhibited angiogenesis in nonretinal models such as the hind limb ischemia model. Our work demonstrates that Nutlin-3 functions through an antiproliferative pathway with conceivable advantages over existing cytokinetargeted antiangiogenesis therapies.

Original languageEnglish
Pages (from-to)4170-4181
Number of pages12
JournalJournal of Clinical Investigation
Volume123
Issue number10
DOIs
StatePublished - 1 Oct 2013

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    Chavala, S. H., Kim, Y., Tudisco, L., Cicatiello, V., Milde, T., Kerur, N., Claros, N., Yanni, S., Guaiquil, V. H., Hauswirth, W. W., Penn, J. S., Rafii, S., Falco, S. D., Lee, T. C., & Ambati, J. (2013). Retinal angiogenesis suppression through small molecule activation of p53. Journal of Clinical Investigation, 123(10), 4170-4181. https://doi.org/10.1172/JCI67315