TY - JOUR
T1 - Results of a collaborative study on DNA identification of aged bone samples
AU - Vanek, Daniel
AU - Budowle, Bruce
AU - Dubska-Votrubova, Jitka
AU - Ambers, Angie
AU - Frolik, Jan
AU - Pospisek, Martin
AU - Al Afeefi, Ahmed Anwar
AU - Al Hosani, Khalid Ismaeil
AU - Allen, Marie
AU - Al Naimi, Khudooma Saeed
AU - Al Salafi, Dina
AU - Al Tayyari, Wafa Ali Rashid
AU - Arguetaa, Wendy
AU - Bottinelli, Michel
AU - Bus, Magdalena M.
AU - Cemper-Kiesslich, Jan
AU - Cepil, Olivier
AU - De Cock, Greet
AU - Desmyter, Stijn
AU - El Amri, Hamid
AU - El Ossmani, Hicham
AU - Galdies, Ruth
AU - Grün, Sebastian
AU - Guidet, Francois
AU - Hoefges, Anna
AU - Iancu, Cristian Bogdan
AU - Lotz, Petra
AU - Maresca, Alessandro
AU - Nagy, Marion
AU - Novotny, Jindrich
AU - Rachid, Hajar
AU - Rothe, Jessica
AU - Stenersen, Marguerethe
AU - Stephenson, Mishel
AU - Stevanovitch, Alain
AU - Strien, Juliane
AU - Sumita, Denilce R.
AU - Vella, Joanna
AU - Zander, Judith
PY - 2017
Y1 - 2017
N2 - Aim A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. Methods Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. Results Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. Conclusion The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.
AB - Aim A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. Methods Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. Results Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. Conclusion The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.
UR - http://www.scopus.com/inward/record.url?scp=85020554543&partnerID=8YFLogxK
U2 - 10.3325/cmj.2017.58.203
DO - 10.3325/cmj.2017.58.203
M3 - Article
C2 - 28613037
AN - SCOPUS:85020554543
VL - 58
SP - 203
EP - 213
JO - Croatian Medical Journal
JF - Croatian Medical Journal
SN - 0353-9504
IS - 3
ER -