Results of a collaborative study on DNA identification of aged bone samples

Daniel Vanek, Bruce Budowle, Jitka Dubska-Votrubova, Angie Ambers, Jan Frolik, Martin Pospisek, Ahmed Anwar Al Afeefi, Khalid Ismaeil Al Hosani, Marie Allen, Khudooma Saeed Al Naimi, Dina Al Salafi, Wafa Ali Rashid Al Tayyari, Wendy Arguetaa, Michel Bottinelli, Magdalena M. Bus, Jan Cemper-Kiesslich, Olivier Cepil, Greet De Cock, Stijn Desmyter, Hamid El AmriHicham El Ossmani, Ruth Galdies, Sebastian Grün, Francois Guidet, Anna Hoefges, Cristian Bogdan Iancu, Petra Lotz, Alessandro Maresca, Marion Nagy, Jindrich Novotny, Hajar Rachid, Jessica Rothe, Marguerethe Stenersen, Mishel Stephenson, Alain Stevanovitch, Juliane Strien, Denilce R. Sumita, Joanna Vella, Judith Zander

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Aim A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. Methods Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. Results Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. Conclusion The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.

Original languageEnglish
Pages (from-to)203-213
Number of pages11
JournalCroatian Medical Journal
Volume58
Issue number3
DOIs
StatePublished - 2017

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    Vanek, D., Budowle, B., Dubska-Votrubova, J., Ambers, A., Frolik, J., Pospisek, M., Al Afeefi, A. A., Al Hosani, K. I., Allen, M., Al Naimi, K. S., Al Salafi, D., Al Tayyari, W. A. R., Arguetaa, W., Bottinelli, M., Bus, M. M., Cemper-Kiesslich, J., Cepil, O., De Cock, G., Desmyter, S., ... Zander, J. (2017). Results of a collaborative study on DNA identification of aged bone samples. Croatian Medical Journal, 58(3), 203-213. https://doi.org/10.3325/cmj.2017.58.203