Abstract
We measured steady state and time-resolved resonance energy transfer between donors and acceptors in model membranes. The donor was a long lifetime rhenium-lipid complex, which displayed a mean lifetime of 1 μs and lifetime components as long as 3 μs in the labeled DOPC membranes. The transfer efficiencies were found to be substantially larger than those predicted without consideration of lateral diffusion. The larger transfer efficiencies are consistent with a mutual lateral diffusion coefficient in the membrane near 2x10-8 cm2/s. These results demonstrate that lateral diffusion in membranes can be detected with μs lipid probes. Copyright (C) 1999 Elsevier Science Ireland Ltd.
Original language | English |
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Pages (from-to) | 243-253 |
Number of pages | 11 |
Journal | Chemistry and Physics of Lipids |
Volume | 101 |
Issue number | 2 |
DOIs | |
State | Published - Sep 1999 |
Keywords
- Metal-ligand lipid conjugate
- Model membrane
- Resonance energy transfer