Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane

Li Li, Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

We measured steady state and time-resolved resonance energy transfer between donors and acceptors in model membranes. The donor was a long lifetime rhenium-lipid complex, which displayed a mean lifetime of 1 μs and lifetime components as long as 3 μs in the labeled DOPC membranes. The transfer efficiencies were found to be substantially larger than those predicted without consideration of lateral diffusion. The larger transfer efficiencies are consistent with a mutual lateral diffusion coefficient in the membrane near 2x10-8 cm2/s. These results demonstrate that lateral diffusion in membranes can be detected with μs lipid probes. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)243-253
Number of pages11
JournalChemistry and Physics of Lipids
Volume101
Issue number2
DOIs
StatePublished - Sep 1999

Keywords

  • Metal-ligand lipid conjugate
  • Model membrane
  • Resonance energy transfer

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