Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane

Li Li; Ignacy Gryczynski; Joseph R. Lakowicz

doi:10.1016/S0009-3084(99)00066-3

Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane

Li Li, Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We measured steady state and time-resolved resonance energy transfer between donors and acceptors in model membranes. The donor was a long lifetime rhenium-lipid complex, which displayed a mean lifetime of 1 μs and lifetime components as long as 3 μs in the labeled DOPC membranes. The transfer efficiencies were found to be substantially larger than those predicted without consideration of lateral diffusion. The larger transfer efficiencies are consistent with a mutual lateral diffusion coefficient in the membrane near 2x10^-8 cm²/s. These results demonstrate that lateral diffusion in membranes can be detected with μs lipid probes. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	243-253
Number of pages	11
Journal	Chemistry and Physics of Lipids
Volume	101
Issue number	2
DOIs	https://doi.org/10.1016/S0009-3084(99)00066-3
State	Published - Sep 1999

Keywords

Metal-ligand lipid conjugate
Model membrane
Resonance energy transfer

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10.1016/S0009-3084(99)00066-3

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title = "Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane",

abstract = "We measured steady state and time-resolved resonance energy transfer between donors and acceptors in model membranes. The donor was a long lifetime rhenium-lipid complex, which displayed a mean lifetime of 1 μs and lifetime components as long as 3 μs in the labeled DOPC membranes. The transfer efficiencies were found to be substantially larger than those predicted without consideration of lateral diffusion. The larger transfer efficiencies are consistent with a mutual lateral diffusion coefficient in the membrane near 2x10-8 cm2/s. These results demonstrate that lateral diffusion in membranes can be detected with μs lipid probes. Copyright (C) 1999 Elsevier Science Ireland Ltd.",

keywords = "Metal-ligand lipid conjugate, Model membrane, Resonance energy transfer",

author = "Li Li and Ignacy Gryczynski and Lakowicz, {Joseph R.}",

note = "Funding Information: This work was supported by the NIH, National Center for Research Resources PN-08119, and by the National Institute for General Medical Sciences, GM-35154.",

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T1 - Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane

AU - Li, Li

AU - Gryczynski, Ignacy

AU - Lakowicz, Joseph R.

N1 - Funding Information: This work was supported by the NIH, National Center for Research Resources PN-08119, and by the National Institute for General Medical Sciences, GM-35154.

PY - 1999/9

Y1 - 1999/9

N2 - We measured steady state and time-resolved resonance energy transfer between donors and acceptors in model membranes. The donor was a long lifetime rhenium-lipid complex, which displayed a mean lifetime of 1 μs and lifetime components as long as 3 μs in the labeled DOPC membranes. The transfer efficiencies were found to be substantially larger than those predicted without consideration of lateral diffusion. The larger transfer efficiencies are consistent with a mutual lateral diffusion coefficient in the membrane near 2x10-8 cm2/s. These results demonstrate that lateral diffusion in membranes can be detected with μs lipid probes. Copyright (C) 1999 Elsevier Science Ireland Ltd.

AB - We measured steady state and time-resolved resonance energy transfer between donors and acceptors in model membranes. The donor was a long lifetime rhenium-lipid complex, which displayed a mean lifetime of 1 μs and lifetime components as long as 3 μs in the labeled DOPC membranes. The transfer efficiencies were found to be substantially larger than those predicted without consideration of lateral diffusion. The larger transfer efficiencies are consistent with a mutual lateral diffusion coefficient in the membrane near 2x10-8 cm2/s. These results demonstrate that lateral diffusion in membranes can be detected with μs lipid probes. Copyright (C) 1999 Elsevier Science Ireland Ltd.

KW - Metal-ligand lipid conjugate

KW - Model membrane

KW - Resonance energy transfer

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U2 - 10.1016/S0009-3084(99)00066-3

DO - 10.1016/S0009-3084(99)00066-3

M3 - Article

C2 - 10533265

AN - SCOPUS:0032791264

VL - 101

SP - 243

EP - 253

JO - Chemistry and Physics of Lipids

JF - Chemistry and Physics of Lipids

SN - 0009-3084

IS - 2

ER -

Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane

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