Resonance energy transfer between fluorescent BSA protected Au nanoclusters and organic fluorophores

Sangram Limbaji Raut, Ryan Rich, Rafal Fudala, Susan Butler, Rutika Kokate, Zygmunt Gryczynski, Rafal Luchowski, Ignacy Gryczynski

Research output: Contribution to journalArticleResearchpeer-review

39 Citations (Scopus)

Abstract

Bovine serum albumin (BSA) protected nanoclusters (Au and Ag) represent a group of nanomaterials that holds great promise in biophysical applications due to their unique fluorescence properties and lack of toxicity. These metal nanoclusters have utility in a variety of disciplines including catalysis, biosensing, photonics, imaging and molecular electronics. However, they suffer from several disadvantages such as low fluorescence quantum efficiency (typically near 6%) and broad emission spectrum (540 nm to 800 nm). We describe an approach to enhance the apparent brightness of BSA Au clusters by linking them with a high extinction donor organic dye pacific blue (PB). In this conjugate PB acts as a donor to BSA Au clusters and enhances its brightness by resonance energy transfer (RET). We found that the emission of BSA Au clusters can be enhanced by a magnitude of two-fold by resonance energy transfer (RET) from the high extinction donor PB, and BSA Au clusters can act as an acceptor to nanosecond lifetime organic dyes. By pumping the BSA Au clusters using a high extinction donor, one can increase the effective brightness of less bright fluorophores like BSA Au clusters. Moreover, we prepared another conjugate of BSA Au clusters with the near infrared (NIR) dye Dylight 750 (Dy750), where BSA Au clusters act as a donor to Dy750. We observed that BSA Au clusters can function as a donor, showing 46% transfer efficiency to the NIR dye Dy750 with a long lifetime component in the acceptor decay through RET. Such RET-based probes can be used to prevent the problems of a broad emission spectrum associated with the BSA Au clusters. Moreover, transferring energy from BSA Au clusters to Dy750 will result in a RET probe with a narrow emission spectrum and long lifetime component which can be utilized in imaging applications.

Original languageEnglish
Pages (from-to)385-391
Number of pages7
JournalNanoscale
Volume6
Issue number1
DOIs
StatePublished - 7 Jan 2014

Fingerprint

Fluorophores
Nanoclusters
Bovine Serum Albumin
Energy transfer
Dyes
Luminance
Fluorescence
Infrared radiation
Coloring Agents
Imaging techniques
Molecular electronics
Quantum efficiency
Nanostructured materials
Photonics
Catalysis
Toxicity
Metals

Cite this

Raut, Sangram Limbaji ; Rich, Ryan ; Fudala, Rafal ; Butler, Susan ; Kokate, Rutika ; Gryczynski, Zygmunt ; Luchowski, Rafal ; Gryczynski, Ignacy. / Resonance energy transfer between fluorescent BSA protected Au nanoclusters and organic fluorophores. In: Nanoscale. 2014 ; Vol. 6, No. 1. pp. 385-391.
@article{a09cdd40634e4e2bb6f93974db0cb29b,
title = "Resonance energy transfer between fluorescent BSA protected Au nanoclusters and organic fluorophores",
abstract = "Bovine serum albumin (BSA) protected nanoclusters (Au and Ag) represent a group of nanomaterials that holds great promise in biophysical applications due to their unique fluorescence properties and lack of toxicity. These metal nanoclusters have utility in a variety of disciplines including catalysis, biosensing, photonics, imaging and molecular electronics. However, they suffer from several disadvantages such as low fluorescence quantum efficiency (typically near 6{\%}) and broad emission spectrum (540 nm to 800 nm). We describe an approach to enhance the apparent brightness of BSA Au clusters by linking them with a high extinction donor organic dye pacific blue (PB). In this conjugate PB acts as a donor to BSA Au clusters and enhances its brightness by resonance energy transfer (RET). We found that the emission of BSA Au clusters can be enhanced by a magnitude of two-fold by resonance energy transfer (RET) from the high extinction donor PB, and BSA Au clusters can act as an acceptor to nanosecond lifetime organic dyes. By pumping the BSA Au clusters using a high extinction donor, one can increase the effective brightness of less bright fluorophores like BSA Au clusters. Moreover, we prepared another conjugate of BSA Au clusters with the near infrared (NIR) dye Dylight 750 (Dy750), where BSA Au clusters act as a donor to Dy750. We observed that BSA Au clusters can function as a donor, showing 46{\%} transfer efficiency to the NIR dye Dy750 with a long lifetime component in the acceptor decay through RET. Such RET-based probes can be used to prevent the problems of a broad emission spectrum associated with the BSA Au clusters. Moreover, transferring energy from BSA Au clusters to Dy750 will result in a RET probe with a narrow emission spectrum and long lifetime component which can be utilized in imaging applications.",
author = "Raut, {Sangram Limbaji} and Ryan Rich and Rafal Fudala and Susan Butler and Rutika Kokate and Zygmunt Gryczynski and Rafal Luchowski and Ignacy Gryczynski",
year = "2014",
month = "1",
day = "7",
doi = "10.1039/c3nr03886f",
language = "English",
volume = "6",
pages = "385--391",
journal = "Nanoscale",
issn = "2040-3364",
publisher = "Royal Society of Chemistry",
number = "1",

}

Resonance energy transfer between fluorescent BSA protected Au nanoclusters and organic fluorophores. / Raut, Sangram Limbaji; Rich, Ryan; Fudala, Rafal; Butler, Susan; Kokate, Rutika; Gryczynski, Zygmunt; Luchowski, Rafal; Gryczynski, Ignacy.

In: Nanoscale, Vol. 6, No. 1, 07.01.2014, p. 385-391.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Resonance energy transfer between fluorescent BSA protected Au nanoclusters and organic fluorophores

AU - Raut, Sangram Limbaji

AU - Rich, Ryan

AU - Fudala, Rafal

AU - Butler, Susan

AU - Kokate, Rutika

AU - Gryczynski, Zygmunt

AU - Luchowski, Rafal

AU - Gryczynski, Ignacy

PY - 2014/1/7

Y1 - 2014/1/7

N2 - Bovine serum albumin (BSA) protected nanoclusters (Au and Ag) represent a group of nanomaterials that holds great promise in biophysical applications due to their unique fluorescence properties and lack of toxicity. These metal nanoclusters have utility in a variety of disciplines including catalysis, biosensing, photonics, imaging and molecular electronics. However, they suffer from several disadvantages such as low fluorescence quantum efficiency (typically near 6%) and broad emission spectrum (540 nm to 800 nm). We describe an approach to enhance the apparent brightness of BSA Au clusters by linking them with a high extinction donor organic dye pacific blue (PB). In this conjugate PB acts as a donor to BSA Au clusters and enhances its brightness by resonance energy transfer (RET). We found that the emission of BSA Au clusters can be enhanced by a magnitude of two-fold by resonance energy transfer (RET) from the high extinction donor PB, and BSA Au clusters can act as an acceptor to nanosecond lifetime organic dyes. By pumping the BSA Au clusters using a high extinction donor, one can increase the effective brightness of less bright fluorophores like BSA Au clusters. Moreover, we prepared another conjugate of BSA Au clusters with the near infrared (NIR) dye Dylight 750 (Dy750), where BSA Au clusters act as a donor to Dy750. We observed that BSA Au clusters can function as a donor, showing 46% transfer efficiency to the NIR dye Dy750 with a long lifetime component in the acceptor decay through RET. Such RET-based probes can be used to prevent the problems of a broad emission spectrum associated with the BSA Au clusters. Moreover, transferring energy from BSA Au clusters to Dy750 will result in a RET probe with a narrow emission spectrum and long lifetime component which can be utilized in imaging applications.

AB - Bovine serum albumin (BSA) protected nanoclusters (Au and Ag) represent a group of nanomaterials that holds great promise in biophysical applications due to their unique fluorescence properties and lack of toxicity. These metal nanoclusters have utility in a variety of disciplines including catalysis, biosensing, photonics, imaging and molecular electronics. However, they suffer from several disadvantages such as low fluorescence quantum efficiency (typically near 6%) and broad emission spectrum (540 nm to 800 nm). We describe an approach to enhance the apparent brightness of BSA Au clusters by linking them with a high extinction donor organic dye pacific blue (PB). In this conjugate PB acts as a donor to BSA Au clusters and enhances its brightness by resonance energy transfer (RET). We found that the emission of BSA Au clusters can be enhanced by a magnitude of two-fold by resonance energy transfer (RET) from the high extinction donor PB, and BSA Au clusters can act as an acceptor to nanosecond lifetime organic dyes. By pumping the BSA Au clusters using a high extinction donor, one can increase the effective brightness of less bright fluorophores like BSA Au clusters. Moreover, we prepared another conjugate of BSA Au clusters with the near infrared (NIR) dye Dylight 750 (Dy750), where BSA Au clusters act as a donor to Dy750. We observed that BSA Au clusters can function as a donor, showing 46% transfer efficiency to the NIR dye Dy750 with a long lifetime component in the acceptor decay through RET. Such RET-based probes can be used to prevent the problems of a broad emission spectrum associated with the BSA Au clusters. Moreover, transferring energy from BSA Au clusters to Dy750 will result in a RET probe with a narrow emission spectrum and long lifetime component which can be utilized in imaging applications.

UR - http://www.scopus.com/inward/record.url?scp=84890209885&partnerID=8YFLogxK

U2 - 10.1039/c3nr03886f

DO - 10.1039/c3nr03886f

M3 - Article

VL - 6

SP - 385

EP - 391

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 1

ER -