Resolution of the conformational distribution and dynamics of a flexible molecule using frequency-domain fluorometry

Joseph R. Lakowicz, Józef Kuśba, Wieslaw Wiczk, Ignacy GryczynSki, Henryk Szmacinski, Michael L. Johnson

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

We report the first resolution of both the conformational distribution and end-to-end diffusion coefficient of a flexible molecule. This molecular information was recovered using only the donor intensity decay in a single solvent at a single viscosity, as observed by the technique of frequency-domain fluorometry. This technique can be extended to measurements of structural fluctuations of biological macromolecules.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalBiophysical Chemistry
Volume39
Issue number1
DOIs
StatePublished - Jan 1991

Keywords

  • Conformational analysis
  • Fluorescence
  • Frequency-domain fluorometry
  • Molecular dynamics
  • Resonance energy transfer

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