Abstract
We measured the wavelength-dependent intensity decays of 4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazo-1,2a-purine (Yt-base) in propanol to determine the time-resolved emission spectra and rates of spectral relaxation. We found that resolution of the spectral relaxation times was dramatically improved by global analysis of the frequency-domain data with increasing amounts of the collisional quencher CCl4. Collisional quenching preferentially decreases the longer-lived relaxed component of the emission, thereby increasing the fractional contribution of the incompletely relaxed portion of the emission. The data could not be explained by a single spectral relaxation time, and at least two relaxation times are needed to describe the time-dependent emission center of gravity of Yt-base.
Original language | English |
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Pages (from-to) | 177-185 |
Number of pages | 9 |
Journal | Journal of Fluorescence |
Volume | 6 |
Issue number | 3 |
DOIs | |
State | Published - 1 Jan 1996 |
Keywords
- Fluorescence decay
- Frequency-domain fluorometry
- Solvent effects
- Solvent relaxation
- Time-resolved emission spectra