Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-Marked Lentiviral Vectors in the Chemically Defined Medium

Zhicheng Shao, Ricardo Raúl Cevallos, Kejin Hu

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

Much investigation is needed to understand the underlying molecular mechanisms of iPSC reprogramming and to improve this technology. Lentivirus-mediated iPSC reprogramming remains the most effective method to study human pluripotency reprogramming. iPSC production is more efficient and consistent in the chemically defined medium. Fibroblasts are the most common starting cells for iPSC generation. Here, we provide a detailed protocol for iPSC generation from human fibroblasts using the GFP-expressing lentiviral vectors in the chemically defined medium.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages101-116
Number of pages16
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2239
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Chemically defined medium
  • Co-expression of GFP
  • Fibroblasts
  • Human induced pluripotent stem cells
  • Human iPSC
  • Lentiviral vectors
  • Pluripotent reprogramming

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