@inbook{2388cde07bfb4e379757648c1663db9e,
title = "Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-Marked Lentiviral Vectors in the Chemically Defined Medium",
abstract = "Much investigation is needed to understand the underlying molecular mechanisms of iPSC reprogramming and to improve this technology. Lentivirus-mediated iPSC reprogramming remains the most effective method to study human pluripotency reprogramming. iPSC production is more efficient and consistent in the chemically defined medium. Fibroblasts are the most common starting cells for iPSC generation. Here, we provide a detailed protocol for iPSC generation from human fibroblasts using the GFP-expressing lentiviral vectors in the chemically defined medium.",
keywords = "Chemically defined medium, Co-expression of GFP, Fibroblasts, Human induced pluripotent stem cells, Human iPSC, Lentiviral vectors, Pluripotent reprogramming",
author = "Zhicheng Shao and Cevallos, {Ricardo Ra{\'u}l} and Kejin Hu",
note = "Funding Information: The research in Hu laboratory is supported by NIH (1R01GM127411-01A1) and the American Heart Association (17GRNT33670780). Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1084-8_7",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "101--116",
booktitle = "Methods in Molecular Biology",
}