Renal protection of in vivo administration of tempol in streptozotocin-induced diabetic rats

Jiajie Luan, Weiping Li, Jia Han, Wen Zhang, Huiling Gong, Rong Ma

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


The present study was carried out to investigate the protective effects of tempol on renal function and the underlying mechanism in streptozotocin-induced diabetic rats. The diabetic rats were randomly divided into the model group (without tempol) and tempol group (1 mM tempol in drinking water for 6 weeks). Nondiabetic rats were served as the Control group. The mRNA expression of canonical transient receptor potential 6 (TRPC6), transforming growth factor (TGF)-β1, and type IV collagen (Col IV) were examined. The malondialdehyde (MDA) level, activities of superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in renal tissues were measured to assess redox status in kidneys. We found that tempol significantly reduced 24-h urine output and urine albuminuria excretion in the diabetic rats. Compared with the model group, the concentration of MDA was significantly lower in the tempol group. In addition, diabetes decreased activities of SOD and GSH-Px and these responses were prevented by tempol treatment. Moreover, in diabetic rats, the mRNA expression levels of TGF-β1 and Col IV were upregulated. TRPC6 mRNA expression level was down-regulated in diabetic kidneys. However, all of these diabetic effects were significantly suppressed by tempol treatment. These results suggest that chronic treatment of diabetic rats with tempol can protect kidneys, possibly by reducing expression of TGF-β1, Col IV, and upregulating TRPC6 expression level.

Original languageEnglish
Pages (from-to)167-176
Number of pages10
JournalJournal of Pharmacological Sciences
Issue number2
StatePublished - 2012


  • Collagen IV
  • Diabetic nephropathy
  • Tempol
  • Transient receptor potential 6 (TRPC6)


Dive into the research topics of 'Renal protection of in vivo administration of tempol in streptozotocin-induced diabetic rats'. Together they form a unique fingerprint.

Cite this