TY - JOUR
T1 - Remodeling of phosphatidylglycerol in Synechocystis PCC6803
AU - Laczko-Dobos, Hajnalka
AU - Fryčák, Petr
AU - Ughy, Bettina
AU - Domonkos, Ildiko
AU - Wada, Hajime
AU - Prokai, Laszlo
AU - Gombos, Zoltan
PY - 2010/2/1
Y1 - 2010/2/1
N2 - The phosphatidylglycerol deficient ΔpgsA mutant of Synechocystis PCC6803 provided a unique experimental system for investigating in vivo retailoring of exogenously added dioleoylphosphatidylglycerol in phosphatidylglycerol-depleted cells. Gas chromatographic analysis of fatty acid composition suggested that diacyl-phosphatidylglycerols were synthesized from the artificial synthetic precursor. The formation of new, retailored lipid species was confirmed by negative-ion electrospray ionization-Fourier-transform ion cyclotron resonance and ion trap tandem mass spectrometry. Various isomeric diacyl-phosphatidylglycerols were identified indicating transesterification of the exogenously added dioleoylphosphatidyl-glycerol at the sn-1 or sn-2 positions. Polyunsaturated fatty acids were incorporated selectively into the sn-1 position. Our experiments with Synechocystis PCC6803/ΔpgsA mutant cells demonstrated lipid remodeling in a prokaryotic photosynthetic bacterium. Our data suggest that the remodeling of diacylphosphatidylglycerol likely involves reactions catalyzed by phospholipase A1 and A2 or acyl-hydrolase, lysophosphatidylglycerol acyltransferase and acyl-lipid desaturases.
AB - The phosphatidylglycerol deficient ΔpgsA mutant of Synechocystis PCC6803 provided a unique experimental system for investigating in vivo retailoring of exogenously added dioleoylphosphatidylglycerol in phosphatidylglycerol-depleted cells. Gas chromatographic analysis of fatty acid composition suggested that diacyl-phosphatidylglycerols were synthesized from the artificial synthetic precursor. The formation of new, retailored lipid species was confirmed by negative-ion electrospray ionization-Fourier-transform ion cyclotron resonance and ion trap tandem mass spectrometry. Various isomeric diacyl-phosphatidylglycerols were identified indicating transesterification of the exogenously added dioleoylphosphatidyl-glycerol at the sn-1 or sn-2 positions. Polyunsaturated fatty acids were incorporated selectively into the sn-1 position. Our experiments with Synechocystis PCC6803/ΔpgsA mutant cells demonstrated lipid remodeling in a prokaryotic photosynthetic bacterium. Our data suggest that the remodeling of diacylphosphatidylglycerol likely involves reactions catalyzed by phospholipase A1 and A2 or acyl-hydrolase, lysophosphatidylglycerol acyltransferase and acyl-lipid desaturases.
KW - Mass spectrometry
KW - Membrane lipid
KW - Phosphatidylglycerol
KW - Photosynthetic organism
KW - Remodeling
KW - Synechocystis PCC6803
UR - http://www.scopus.com/inward/record.url?scp=73749084716&partnerID=8YFLogxK
U2 - 10.1016/j.bbalip.2009.10.009
DO - 10.1016/j.bbalip.2009.10.009
M3 - Article
C2 - 19857602
AN - SCOPUS:73749084716
VL - 1801
SP - 163
EP - 170
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
SN - 1388-1981
IS - 2
ER -