Remodeling of phosphatidylglycerol in Synechocystis PCC6803

Hajnalka Laczko-Dobos, Petr Fryčák, Bettina Ughy, Ildiko Domonkos, Hajime Wada, Laszlo Prokai, Zoltan Gombos

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The phosphatidylglycerol deficient ΔpgsA mutant of Synechocystis PCC6803 provided a unique experimental system for investigating in vivo retailoring of exogenously added dioleoylphosphatidylglycerol in phosphatidylglycerol-depleted cells. Gas chromatographic analysis of fatty acid composition suggested that diacyl-phosphatidylglycerols were synthesized from the artificial synthetic precursor. The formation of new, retailored lipid species was confirmed by negative-ion electrospray ionization-Fourier-transform ion cyclotron resonance and ion trap tandem mass spectrometry. Various isomeric diacyl-phosphatidylglycerols were identified indicating transesterification of the exogenously added dioleoylphosphatidyl-glycerol at the sn-1 or sn-2 positions. Polyunsaturated fatty acids were incorporated selectively into the sn-1 position. Our experiments with Synechocystis PCC6803/ΔpgsA mutant cells demonstrated lipid remodeling in a prokaryotic photosynthetic bacterium. Our data suggest that the remodeling of diacylphosphatidylglycerol likely involves reactions catalyzed by phospholipase A1 and A2 or acyl-hydrolase, lysophosphatidylglycerol acyltransferase and acyl-lipid desaturases.

Original languageEnglish
Pages (from-to)163-170
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1801
Issue number2
DOIs
StatePublished - 1 Feb 2010

Keywords

  • Mass spectrometry
  • Membrane lipid
  • Phosphatidylglycerol
  • Photosynthetic organism
  • Remodeling
  • Synechocystis PCC6803

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