Regulatory CD4+ CD25+ T cells dampen inflammatory disease in murine mycoplasma pneumonia and promote IL-17 and IFN-γ Responses

Adam N. Odeh, Jerry W. Simecka

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Mycoplasmas cause respiratory diseases characterized by persistent infection and chronic airway inflammation. Mycoplasma lung disease is immunopathologic, with CD4+ Th cells determining both disease severity and resistance to infection. Th2 cell responses promote immunopathology, while Th1 cells confer resistance to infection. However, regulatory CD4+ T cells may also have a role in the pathogenesis of mycoplasma respiratory diseases. We hypothesized Treg cells control the severity of the inflammatory lesions and may also promote persistence of infection. To examine this, BALB/c mice were depleted of CD25+ cells, and had increased disease severity due to Mycoplasma pulmonis infection. Increases in mycoplasma antibody responses and lymphocyte infiltration into lungs also occurred after CD25+ cell depletion. CD4+ CD25+ regulatory T cells promoted IFN-ã and IL-17 mycoplasma-specific CD4+ T cell responses in vitro and in vivo, while dampening IL-13+ Th responses. Neither IL-10 nor TGF-β expression was detected in CD4+ CD25+ T cells from lymph nodes. Thus, a regulatory T cell population plays an important role in controlling damaging immune responses in mycoplasma respiratory disease but does not contribute to persistence of infection. It appears that a regulatory T cell population preferentially dampens Th2 cell-mediated inflammatory responses to mycoplasma through a mechanism independent of IL-10 or TGF-β characteristic of "classic" Treg cells.

Original languageEnglish
Article numbere0155648
JournalPLoS ONE
Volume11
Issue number5
DOIs
StatePublished - May 2016

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Mycoplasma pneumoniae
Mycoplasma Pneumonia
T-cells
Interleukin-17
Mycoplasma
Regulatory T-Lymphocytes
Pulmonary diseases
T-lymphocytes
T-Lymphocytes
mice
respiratory tract diseases
Infection
infection
Th2 Cells
cells
Interleukin-10
interleukin-10
disease severity
Mycoplasma pulmonis
Interleukin-13

Cite this

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abstract = "Mycoplasmas cause respiratory diseases characterized by persistent infection and chronic airway inflammation. Mycoplasma lung disease is immunopathologic, with CD4+ Th cells determining both disease severity and resistance to infection. Th2 cell responses promote immunopathology, while Th1 cells confer resistance to infection. However, regulatory CD4+ T cells may also have a role in the pathogenesis of mycoplasma respiratory diseases. We hypothesized Treg cells control the severity of the inflammatory lesions and may also promote persistence of infection. To examine this, BALB/c mice were depleted of CD25+ cells, and had increased disease severity due to Mycoplasma pulmonis infection. Increases in mycoplasma antibody responses and lymphocyte infiltration into lungs also occurred after CD25+ cell depletion. CD4+ CD25+ regulatory T cells promoted IFN-{\~a} and IL-17 mycoplasma-specific CD4+ T cell responses in vitro and in vivo, while dampening IL-13+ Th responses. Neither IL-10 nor TGF-β expression was detected in CD4+ CD25+ T cells from lymph nodes. Thus, a regulatory T cell population plays an important role in controlling damaging immune responses in mycoplasma respiratory disease but does not contribute to persistence of infection. It appears that a regulatory T cell population preferentially dampens Th2 cell-mediated inflammatory responses to mycoplasma through a mechanism independent of IL-10 or TGF-β characteristic of {"}classic{"} Treg cells.",
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Regulatory CD4+ CD25+ T cells dampen inflammatory disease in murine mycoplasma pneumonia and promote IL-17 and IFN-γ Responses. / Odeh, Adam N.; Simecka, Jerry W.

In: PLoS ONE, Vol. 11, No. 5, e0155648, 05.2016.

Research output: Contribution to journalArticle

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