Regulation of ubiquitin-proteasome system-mediated Tip110 protein degradation by USP15

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

Tip110 is a nuclear protein and has been shown to function in tumor antigenicity, regulation of gene transcription, pre-mRNA splicing, stem cell proliferation and differentiation, and embryonic development. To characterize the in vivo functions of Tip110, a transgene cassette expressing human Tip110 protein (hTip110) was used to generate hTip110 transgenic (Tg) mice. Unexpectedly, only Tip110 mRNA but not Tip110 protein was expressed in Tg MEF and tissues. Treatment of Tg MEF with proteasome inhibitors led to detection of hTip110 protein, which prompted us to investigate the regulatory mechanisms of Tip110 degradation in mouse cells. We found that hTip110 was more sensitive to ubiquitin-proteasome system (UPS)-mediated protein degradation than mouse Tip110 (mTip110), likely resulting from more hTip110 ubiquitination. Using affinity chromatography and proteomics, we identified USP15, a deubiquitinating enzyme, to be associated with Tip110. Tip110 expression led to re-distribution of USP15 from the cytoplasm to the nucleus and complete co-localization of Tip110 with USP15 in the nucleus, whereas USP15 expression resulted in hTip110 deubiquitination. Interestingly, USP15 knockdown restored hTip110 protein expression in Tg MEF and USP15 expression had little effects. Taken together, these results provide insights into the regulatory mechanism of human Tip110 degradation by USP15.

Original languageEnglish
Pages (from-to)10-19
Number of pages10
JournalInternational Journal of Biochemistry and Cell Biology
Volume54
DOIs
StatePublished - 1 Jan 2014

Fingerprint

Proteasome Endopeptidase Complex
Ubiquitin
Proteolysis
Degradation
Proteins
Affinity chromatography
Proteasome Inhibitors
Ubiquitination
RNA Precursors
Cell proliferation
Transcription
Nuclear Proteins
human SART3 protein
Stem cells
Transgenes
Affinity Chromatography
Proteomics
Transgenic Mice
Embryonic Development
Tumors

Keywords

  • Protein degradation
  • Tip110/SART3
  • Ubiquitin proteasome system
  • USP15

Cite this

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title = "Regulation of ubiquitin-proteasome system-mediated Tip110 protein degradation by USP15",
abstract = "Tip110 is a nuclear protein and has been shown to function in tumor antigenicity, regulation of gene transcription, pre-mRNA splicing, stem cell proliferation and differentiation, and embryonic development. To characterize the in vivo functions of Tip110, a transgene cassette expressing human Tip110 protein (hTip110) was used to generate hTip110 transgenic (Tg) mice. Unexpectedly, only Tip110 mRNA but not Tip110 protein was expressed in Tg MEF and tissues. Treatment of Tg MEF with proteasome inhibitors led to detection of hTip110 protein, which prompted us to investigate the regulatory mechanisms of Tip110 degradation in mouse cells. We found that hTip110 was more sensitive to ubiquitin-proteasome system (UPS)-mediated protein degradation than mouse Tip110 (mTip110), likely resulting from more hTip110 ubiquitination. Using affinity chromatography and proteomics, we identified USP15, a deubiquitinating enzyme, to be associated with Tip110. Tip110 expression led to re-distribution of USP15 from the cytoplasm to the nucleus and complete co-localization of Tip110 with USP15 in the nucleus, whereas USP15 expression resulted in hTip110 deubiquitination. Interestingly, USP15 knockdown restored hTip110 protein expression in Tg MEF and USP15 expression had little effects. Taken together, these results provide insights into the regulatory mechanism of human Tip110 degradation by USP15.",
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Regulation of ubiquitin-proteasome system-mediated Tip110 protein degradation by USP15. / Timani, Khalid A.; Liu, Ying; Suvannasankha, Attaya; He, Johnny Jianglin.

In: International Journal of Biochemistry and Cell Biology, Vol. 54, 01.01.2014, p. 10-19.

Research output: Contribution to journalArticleResearchpeer-review

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