Regulation of Na,K-ATPase Expression by Endothelin-1 in Transformed Human Ciliary Non-Pigmented Epithelial (HNPE) Cells

Raghu Krishnamoorthy, Ganesh Prasanna, Rachel Dauphin, Christina Hulet, Neeraj Agarwal, Thomas Yorio

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

Endothelin-1 (ET-1) (1-100 nM) decreases the activity of Na,K-ATPase, a key enzyme responsible for aqueous humor formation, in transformed human non-pigmented ciliary epithelial (HNPE) cells. The present study sought to determine if ET-1 alters the expression of the catalytically active alpha subunit of Na,K-ATPase in HNPE cells and identify mechanisms underlying these effects. We report that acute (15 and 30 min) treatment with ET-1 results in an increase in mRNA expression of the α1 subunit of Na,K-ATPase. Similar to ET-1's effects, ouabain (100 μM), a selective inhibitor of Na,K-ATPase, and monensin (10 μM), a sodium ionophore, also increased Na,K-ATPase expression in HNPE cells. The increase in Na,K-ATPase expression by short-term treatment with ouabain and monensin was dependent on their ability to elevate intracellular sodium concentrations. However, acute ET-1 treatment mediated increase in Na,K-ATPase expression was independent of changes in intracellular sodium. A prolonged (24 hr) ET-1 treatment results in an increase in both mRNA and protein levels of the α1 subunit of Na,K-ATPase. These observations suggest that ET-1 could play a homeostatic role in modulating aqueous humor formation by having differential effects on the activity and expression of Na,K-ATPase by the ciliary epithelium in the eye.

Original languageEnglish
Pages (from-to)465-481
Number of pages17
JournalJournal of Ocular Pharmacology and Therapeutics
Volume19
Issue number5
DOIs
StatePublished - 1 Jan 2003

Fingerprint

Endothelin-1
Epithelial Cells
Monensin
Aqueous Humor
Ouabain
Sodium Ionophores
Sodium
Messenger RNA
sodium-translocating ATPase
Epithelium
Enzymes
Proteins

Cite this

@article{47108860810d44c6b0a852804272a62b,
title = "Regulation of Na,K-ATPase Expression by Endothelin-1 in Transformed Human Ciliary Non-Pigmented Epithelial (HNPE) Cells",
abstract = "Endothelin-1 (ET-1) (1-100 nM) decreases the activity of Na,K-ATPase, a key enzyme responsible for aqueous humor formation, in transformed human non-pigmented ciliary epithelial (HNPE) cells. The present study sought to determine if ET-1 alters the expression of the catalytically active alpha subunit of Na,K-ATPase in HNPE cells and identify mechanisms underlying these effects. We report that acute (15 and 30 min) treatment with ET-1 results in an increase in mRNA expression of the α1 subunit of Na,K-ATPase. Similar to ET-1's effects, ouabain (100 μM), a selective inhibitor of Na,K-ATPase, and monensin (10 μM), a sodium ionophore, also increased Na,K-ATPase expression in HNPE cells. The increase in Na,K-ATPase expression by short-term treatment with ouabain and monensin was dependent on their ability to elevate intracellular sodium concentrations. However, acute ET-1 treatment mediated increase in Na,K-ATPase expression was independent of changes in intracellular sodium. A prolonged (24 hr) ET-1 treatment results in an increase in both mRNA and protein levels of the α1 subunit of Na,K-ATPase. These observations suggest that ET-1 could play a homeostatic role in modulating aqueous humor formation by having differential effects on the activity and expression of Na,K-ATPase by the ciliary epithelium in the eye.",
author = "Raghu Krishnamoorthy and Ganesh Prasanna and Rachel Dauphin and Christina Hulet and Neeraj Agarwal and Thomas Yorio",
year = "2003",
month = "1",
day = "1",
doi = "10.1089/108076803322473024",
language = "English",
volume = "19",
pages = "465--481",
journal = "Journal of Ocular Pharmacology and Therapeutics",
issn = "1080-7683",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

Regulation of Na,K-ATPase Expression by Endothelin-1 in Transformed Human Ciliary Non-Pigmented Epithelial (HNPE) Cells. / Krishnamoorthy, Raghu; Prasanna, Ganesh; Dauphin, Rachel; Hulet, Christina; Agarwal, Neeraj; Yorio, Thomas.

In: Journal of Ocular Pharmacology and Therapeutics, Vol. 19, No. 5, 01.01.2003, p. 465-481.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Regulation of Na,K-ATPase Expression by Endothelin-1 in Transformed Human Ciliary Non-Pigmented Epithelial (HNPE) Cells

AU - Krishnamoorthy, Raghu

AU - Prasanna, Ganesh

AU - Dauphin, Rachel

AU - Hulet, Christina

AU - Agarwal, Neeraj

AU - Yorio, Thomas

PY - 2003/1/1

Y1 - 2003/1/1

N2 - Endothelin-1 (ET-1) (1-100 nM) decreases the activity of Na,K-ATPase, a key enzyme responsible for aqueous humor formation, in transformed human non-pigmented ciliary epithelial (HNPE) cells. The present study sought to determine if ET-1 alters the expression of the catalytically active alpha subunit of Na,K-ATPase in HNPE cells and identify mechanisms underlying these effects. We report that acute (15 and 30 min) treatment with ET-1 results in an increase in mRNA expression of the α1 subunit of Na,K-ATPase. Similar to ET-1's effects, ouabain (100 μM), a selective inhibitor of Na,K-ATPase, and monensin (10 μM), a sodium ionophore, also increased Na,K-ATPase expression in HNPE cells. The increase in Na,K-ATPase expression by short-term treatment with ouabain and monensin was dependent on their ability to elevate intracellular sodium concentrations. However, acute ET-1 treatment mediated increase in Na,K-ATPase expression was independent of changes in intracellular sodium. A prolonged (24 hr) ET-1 treatment results in an increase in both mRNA and protein levels of the α1 subunit of Na,K-ATPase. These observations suggest that ET-1 could play a homeostatic role in modulating aqueous humor formation by having differential effects on the activity and expression of Na,K-ATPase by the ciliary epithelium in the eye.

AB - Endothelin-1 (ET-1) (1-100 nM) decreases the activity of Na,K-ATPase, a key enzyme responsible for aqueous humor formation, in transformed human non-pigmented ciliary epithelial (HNPE) cells. The present study sought to determine if ET-1 alters the expression of the catalytically active alpha subunit of Na,K-ATPase in HNPE cells and identify mechanisms underlying these effects. We report that acute (15 and 30 min) treatment with ET-1 results in an increase in mRNA expression of the α1 subunit of Na,K-ATPase. Similar to ET-1's effects, ouabain (100 μM), a selective inhibitor of Na,K-ATPase, and monensin (10 μM), a sodium ionophore, also increased Na,K-ATPase expression in HNPE cells. The increase in Na,K-ATPase expression by short-term treatment with ouabain and monensin was dependent on their ability to elevate intracellular sodium concentrations. However, acute ET-1 treatment mediated increase in Na,K-ATPase expression was independent of changes in intracellular sodium. A prolonged (24 hr) ET-1 treatment results in an increase in both mRNA and protein levels of the α1 subunit of Na,K-ATPase. These observations suggest that ET-1 could play a homeostatic role in modulating aqueous humor formation by having differential effects on the activity and expression of Na,K-ATPase by the ciliary epithelium in the eye.

UR - http://www.scopus.com/inward/record.url?scp=0142244526&partnerID=8YFLogxK

U2 - 10.1089/108076803322473024

DO - 10.1089/108076803322473024

M3 - Article

VL - 19

SP - 465

EP - 481

JO - Journal of Ocular Pharmacology and Therapeutics

JF - Journal of Ocular Pharmacology and Therapeutics

SN - 1080-7683

IS - 5

ER -