Regulation of Constitutive Tip110 Expression in Human Cord Blood CD34+ Cells Through Selective Usage of the Proximal and Distal Polyadenylation Sites Within the 3′Untranslated Region

Ying Liu, Xinxin Huang, Khalid A. Timani, Hal E. Broxmeyer, Johnny Jianglin He

Research output: Contribution to journalArticle

Abstract

Tip110 plays important roles for stem cell pluripotency and hematopoiesis. However, little is known about the regulatory mechanisms of Tip110 expression in this process. In this study, we first showed that constitutive Tip110 expression was cell proliferation and differentiation dependent and self-regulated in both human cord blood CD34+ cells. Using a series of molecular techniques, we found that ectopic Tip110 expression led to increased constitutive Tip110 expression through its 3′-untranslated region (3′UTR), specifically through preferential usage of proximal polyadenylation sites within its 3′UTR in cells, including human cord blood CD34+ cells, which indeed led to an increased number of CD34+ cells during differentiation of those cells. Lastly, we showed that Tip110 protein interacted with cleavage stimulation factor 64 (CstF64) protein and that more CstF64 was recruited to the promixal polyadenylation site than the distal polyadenylation site within its 3′UTR. These finding together demonstrates that constitutive Tip110 expression is regulated, at least in part, through its interaction with CstF64, recruitment of CstF64 to, and selective usage of those two polyadenylation sites within its 3′UTR.

Original languageEnglish
Pages (from-to)566-576
Number of pages11
JournalStem Cells and Development
Volume27
Issue number8
DOIs
StatePublished - 15 Apr 2018

Fingerprint

Cleavage Stimulation Factor
Polyadenylation
3' Untranslated Regions
Fetal Blood
Blood Cells
Cell Differentiation
Hematopoiesis
Proteins
Stem Cells
Cell Proliferation

Keywords

  • 3′UTR
  • CD34
  • Tip110
  • hematopoiesis
  • miRNA

Cite this

@article{c8e963e799354722a3bbd892c5f10fae,
title = "Regulation of Constitutive Tip110 Expression in Human Cord Blood CD34+ Cells Through Selective Usage of the Proximal and Distal Polyadenylation Sites Within the 3′Untranslated Region",
abstract = "Tip110 plays important roles for stem cell pluripotency and hematopoiesis. However, little is known about the regulatory mechanisms of Tip110 expression in this process. In this study, we first showed that constitutive Tip110 expression was cell proliferation and differentiation dependent and self-regulated in both human cord blood CD34+ cells. Using a series of molecular techniques, we found that ectopic Tip110 expression led to increased constitutive Tip110 expression through its 3′-untranslated region (3′UTR), specifically through preferential usage of proximal polyadenylation sites within its 3′UTR in cells, including human cord blood CD34+ cells, which indeed led to an increased number of CD34+ cells during differentiation of those cells. Lastly, we showed that Tip110 protein interacted with cleavage stimulation factor 64 (CstF64) protein and that more CstF64 was recruited to the promixal polyadenylation site than the distal polyadenylation site within its 3′UTR. These finding together demonstrates that constitutive Tip110 expression is regulated, at least in part, through its interaction with CstF64, recruitment of CstF64 to, and selective usage of those two polyadenylation sites within its 3′UTR.",
keywords = "3′UTR, CD34, Tip110, hematopoiesis, miRNA",
author = "Ying Liu and Xinxin Huang and Timani, {Khalid A.} and Broxmeyer, {Hal E.} and He, {Johnny Jianglin}",
year = "2018",
month = "4",
day = "15",
doi = "10.1089/scd.2017.0197",
language = "English",
volume = "27",
pages = "566--576",
journal = "Stem Cells and Development",
issn = "1547-3287",
publisher = "Mary Ann Liebert Inc.",
number = "8",

}

TY - JOUR

T1 - Regulation of Constitutive Tip110 Expression in Human Cord Blood CD34+ Cells Through Selective Usage of the Proximal and Distal Polyadenylation Sites Within the 3′Untranslated Region

AU - Liu, Ying

AU - Huang, Xinxin

AU - Timani, Khalid A.

AU - Broxmeyer, Hal E.

AU - He, Johnny Jianglin

PY - 2018/4/15

Y1 - 2018/4/15

N2 - Tip110 plays important roles for stem cell pluripotency and hematopoiesis. However, little is known about the regulatory mechanisms of Tip110 expression in this process. In this study, we first showed that constitutive Tip110 expression was cell proliferation and differentiation dependent and self-regulated in both human cord blood CD34+ cells. Using a series of molecular techniques, we found that ectopic Tip110 expression led to increased constitutive Tip110 expression through its 3′-untranslated region (3′UTR), specifically through preferential usage of proximal polyadenylation sites within its 3′UTR in cells, including human cord blood CD34+ cells, which indeed led to an increased number of CD34+ cells during differentiation of those cells. Lastly, we showed that Tip110 protein interacted with cleavage stimulation factor 64 (CstF64) protein and that more CstF64 was recruited to the promixal polyadenylation site than the distal polyadenylation site within its 3′UTR. These finding together demonstrates that constitutive Tip110 expression is regulated, at least in part, through its interaction with CstF64, recruitment of CstF64 to, and selective usage of those two polyadenylation sites within its 3′UTR.

AB - Tip110 plays important roles for stem cell pluripotency and hematopoiesis. However, little is known about the regulatory mechanisms of Tip110 expression in this process. In this study, we first showed that constitutive Tip110 expression was cell proliferation and differentiation dependent and self-regulated in both human cord blood CD34+ cells. Using a series of molecular techniques, we found that ectopic Tip110 expression led to increased constitutive Tip110 expression through its 3′-untranslated region (3′UTR), specifically through preferential usage of proximal polyadenylation sites within its 3′UTR in cells, including human cord blood CD34+ cells, which indeed led to an increased number of CD34+ cells during differentiation of those cells. Lastly, we showed that Tip110 protein interacted with cleavage stimulation factor 64 (CstF64) protein and that more CstF64 was recruited to the promixal polyadenylation site than the distal polyadenylation site within its 3′UTR. These finding together demonstrates that constitutive Tip110 expression is regulated, at least in part, through its interaction with CstF64, recruitment of CstF64 to, and selective usage of those two polyadenylation sites within its 3′UTR.

KW - 3′UTR

KW - CD34

KW - Tip110

KW - hematopoiesis

KW - miRNA

UR - http://www.scopus.com/inward/record.url?scp=85045855363&partnerID=8YFLogxK

U2 - 10.1089/scd.2017.0197

DO - 10.1089/scd.2017.0197

M3 - Article

C2 - 29583087

AN - SCOPUS:85045855363

VL - 27

SP - 566

EP - 576

JO - Stem Cells and Development

JF - Stem Cells and Development

SN - 1547-3287

IS - 8

ER -