Regulation of autophagy by protein kinase C-ε in breast cancer cells

Research output: Contribution to journalArticle

Abstract

Protein kinase C-ε (PKCε), an anti-apoptotic protein, plays critical roles in breast cancer development and progression. Although autophagy is an important survival mechanism, it is not known if PKCε regulates autophagy in breast cancer cells. We have shown that silencing of PKCε by siRNA inhibited basal and starvation-induced autophagy in T47D breast cancer cells as determined by the decrease in LC3-II, increase in p62, and decrease in autophagy puncta both in the presence and absence of bafilomycin A1. The mechanistic target of rapamycin (mTOR) associates with Raptor or Rictor to form complex-1 (mTORC1) or complex-2 (mTORC2), respectively. Knockdown of PKCε attenuated an increase in autophagy caused by the depletion of Raptor and Rictor. Overexpression of PKCε in MCF-7 cells caused activation of mTORC1 and an increase in LC3-I, LC3-II, and p62. The mTORC1 inhibitor rapamycin abolished the increase in LC3-I and p62. Knockdown of mTOR and Rictor or starvation enhanced autophagy in PKCε overexpressing cells. While overexpression of PKCε in MCF-7 cells inhibited apoptosis, it induced autophagy in response to tumor necrosis factor-α. However, inhibition of autophagy by Atg5 knockdown restored apoptosis in PKCε-overexpressing cells. Thus, PKCε promotes breast cancer cell survival not only by inhibiting apoptosis but also by inducing autophagy.

Original languageEnglish
Article number4247
Pages (from-to)1-11
Number of pages11
JournalInternational journal of molecular sciences
Volume21
Issue number12
DOIs
StatePublished - 2 Jun 2020

Keywords

  • Apoptosis
  • Autophagy
  • Breast cancer
  • MTORC1
  • MTORC2
  • PKCε
  • Raptor
  • Rictor

Fingerprint Dive into the research topics of 'Regulation of autophagy by protein kinase C-ε in breast cancer cells'. Together they form a unique fingerprint.

  • Cite this