Reduction in SIV replication in rhesus macaques infused with autologous lymphocytes engineered with antiviral genes

Robert E. Donahue, Bruch A. Bunnell, M. Christine Zink, Mark E. Metzger, Robert P. Westro, Martha R. Kirby, Tami Unangst, Janice E. Clements, Richard A. Morgan

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Abstract

Simian immunodeficiency virus (SIV) infection of nonhuman primates is one of the most relevant animals models of HIV infection in humans. To test a potential anti-HIV gene therapy strategy in this model, CD4-enriched lymphocytes from three rhesus macaques were subjected to retrovirally mediated gene transfer with a vector expressing an antisense tat/rev gene. This group of animals and three control macaques were subsequently infected with SIV(mac239). Blood and lymph nodes from all macaques were sampled for more than a year to monitor the progress of infection. Although all animals became infected, the animals that received the lymphocytes engineered with the antisense vector demonstrated a significant reduction in viral load in both peripheral blood and lymph nodes, had sustained numbers of CD4+ cells, and exhibited little disruption of lymph node architecture.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalNature Medicine
Volume4
Issue number2
DOIs
StatePublished - 1998

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    Donahue, R. E., Bunnell, B. A., Zink, M. C., Metzger, M. E., Westro, R. P., Kirby, M. R., Unangst, T., Clements, J. E., & Morgan, R. A. (1998). Reduction in SIV replication in rhesus macaques infused with autologous lymphocytes engineered with antiviral genes. Nature Medicine, 4(2), 181-186. https://doi.org/10.1038/nm0298-181