Receptor mediated uptake of paclitaxel from a synthetic high density lipoprotein nanocarrier

Linda K. Mooberry; Maya Nair; Sulabha Paranjape; Walter J. McConathy; Andras G. Lacko

doi:10.3109/10611860903156419

Receptor mediated uptake of paclitaxel from a synthetic high density lipoprotein nanocarrier

Linda K. Mooberry, Maya Nair, Sulabha Paranjape, Walter J. McConathy, Andras G. Lacko

Research output: Contribution to journal › Article › peer-review

111 Scopus citations

Abstract

The purpose of these studies was to determine the mechanism(s) whereby paclitaxel (PTX), is taken up by cancer cells, once encapsulated into synthetic/reconstituted high density lipoprotein (rHDL). The uptake of PTX was found to be facilitated by the scavenger receptor type B-1 (SR-B1) when drug-loaded rHDL particles were incubated with cells that express the SRB1 receptor. Studies with double-labeled, PTX containing rHDL nanoparticles showed that prostate cancer (PC-3) cells incorporated PTX primarily via a selective (SR-B1 type) uptake mechanism. In the presence of a 10-fold excess of plasma HDL, PTX uptake decreased to 30% of the control. These findings suggest that the incorporation of lipophilic drugs by cancer cells from rHDL nanoparticles is facilitated by a receptor mediated (SR-B1) mechanism.

Original language	English
Pages (from-to)	53-58
Number of pages	6
Journal	Journal of Drug Targeting
Volume	18
Issue number	1
DOIs	https://doi.org/10.3109/10611860903156419
State	Published - Jan 2010

Keywords

Folate receptor
Lipid nanoparticles
Lipoprotein
Paclitaxel
Receptor uptake
Targeted drug delivery

Access to Document

10.3109/10611860903156419

Cite this

@article{922c0a9723904b5788928718dc2c1a99,

title = "Receptor mediated uptake of paclitaxel from a synthetic high density lipoprotein nanocarrier",

abstract = "The purpose of these studies was to determine the mechanism(s) whereby paclitaxel (PTX), is taken up by cancer cells, once encapsulated into synthetic/reconstituted high density lipoprotein (rHDL). The uptake of PTX was found to be facilitated by the scavenger receptor type B-1 (SR-B1) when drug-loaded rHDL particles were incubated with cells that express the SRB1 receptor. Studies with double-labeled, PTX containing rHDL nanoparticles showed that prostate cancer (PC-3) cells incorporated PTX primarily via a selective (SR-B1 type) uptake mechanism. In the presence of a 10-fold excess of plasma HDL, PTX uptake decreased to 30% of the control. These findings suggest that the incorporation of lipophilic drugs by cancer cells from rHDL nanoparticles is facilitated by a receptor mediated (SR-B1) mechanism.",

keywords = "Folate receptor, Lipid nanoparticles, Lipoprotein, Paclitaxel, Receptor uptake, Targeted drug delivery",

author = "Mooberry, {Linda K.} and Maya Nair and Sulabha Paranjape and McConathy, {Walter J.} and Lacko, {Andras G.}",

note = "Funding Information: These studies were supported by Cowtown Cruisers for the Cure and a HER grant to AGL from UNTHSC. The ldl A7 and ldl A7 [mSR-BI] cell lines were kindly provided by Dr. Monty Krieger at MIT.",

year = "2010",

month = jan,

doi = "10.3109/10611860903156419",

language = "English",

volume = "18",

pages = "53--58",

journal = "Journal of Drug Targeting",

issn = "1061-186X",

publisher = "Informa Healthcare",

number = "1",

}

TY - JOUR

T1 - Receptor mediated uptake of paclitaxel from a synthetic high density lipoprotein nanocarrier

AU - Mooberry, Linda K.

AU - Nair, Maya

AU - Paranjape, Sulabha

AU - McConathy, Walter J.

AU - Lacko, Andras G.

N1 - Funding Information: These studies were supported by Cowtown Cruisers for the Cure and a HER grant to AGL from UNTHSC. The ldl A7 and ldl A7 [mSR-BI] cell lines were kindly provided by Dr. Monty Krieger at MIT.

PY - 2010/1

Y1 - 2010/1

N2 - The purpose of these studies was to determine the mechanism(s) whereby paclitaxel (PTX), is taken up by cancer cells, once encapsulated into synthetic/reconstituted high density lipoprotein (rHDL). The uptake of PTX was found to be facilitated by the scavenger receptor type B-1 (SR-B1) when drug-loaded rHDL particles were incubated with cells that express the SRB1 receptor. Studies with double-labeled, PTX containing rHDL nanoparticles showed that prostate cancer (PC-3) cells incorporated PTX primarily via a selective (SR-B1 type) uptake mechanism. In the presence of a 10-fold excess of plasma HDL, PTX uptake decreased to 30% of the control. These findings suggest that the incorporation of lipophilic drugs by cancer cells from rHDL nanoparticles is facilitated by a receptor mediated (SR-B1) mechanism.

AB - The purpose of these studies was to determine the mechanism(s) whereby paclitaxel (PTX), is taken up by cancer cells, once encapsulated into synthetic/reconstituted high density lipoprotein (rHDL). The uptake of PTX was found to be facilitated by the scavenger receptor type B-1 (SR-B1) when drug-loaded rHDL particles were incubated with cells that express the SRB1 receptor. Studies with double-labeled, PTX containing rHDL nanoparticles showed that prostate cancer (PC-3) cells incorporated PTX primarily via a selective (SR-B1 type) uptake mechanism. In the presence of a 10-fold excess of plasma HDL, PTX uptake decreased to 30% of the control. These findings suggest that the incorporation of lipophilic drugs by cancer cells from rHDL nanoparticles is facilitated by a receptor mediated (SR-B1) mechanism.

KW - Folate receptor

KW - Lipid nanoparticles

KW - Lipoprotein

KW - Paclitaxel

KW - Receptor uptake

KW - Targeted drug delivery

UR - http://www.scopus.com/inward/record.url?scp=72749122051&partnerID=8YFLogxK

U2 - 10.3109/10611860903156419

DO - 10.3109/10611860903156419

M3 - Article

C2 - 19637935

AN - SCOPUS:72749122051

SN - 1061-186X

VL - 18

SP - 53

EP - 58

JO - Journal of Drug Targeting

JF - Journal of Drug Targeting

IS - 1

ER -

Receptor mediated uptake of paclitaxel from a synthetic high density lipoprotein nanocarrier

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this