Abstract
The purpose of these studies was to determine the mechanism(s) whereby paclitaxel (PTX), is taken up by cancer cells, once encapsulated into synthetic/reconstituted high density lipoprotein (rHDL). The uptake of PTX was found to be facilitated by the scavenger receptor type B-1 (SR-B1) when drug-loaded rHDL particles were incubated with cells that express the SRB1 receptor. Studies with double-labeled, PTX containing rHDL nanoparticles showed that prostate cancer (PC-3) cells incorporated PTX primarily via a selective (SR-B1 type) uptake mechanism. In the presence of a 10-fold excess of plasma HDL, PTX uptake decreased to 30% of the control. These findings suggest that the incorporation of lipophilic drugs by cancer cells from rHDL nanoparticles is facilitated by a receptor mediated (SR-B1) mechanism.
Original language | English |
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Pages (from-to) | 53-58 |
Number of pages | 6 |
Journal | Journal of Drug Targeting |
Volume | 18 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2010 |
Keywords
- Folate receptor
- Lipid nanoparticles
- Lipoprotein
- Paclitaxel
- Receptor uptake
- Targeted drug delivery