Receptor mediated uptake of paclitaxel from a synthetic high density lipoprotein nanocarrier

Linda K. Mooberry, Maya Nair, Sulabha Paranjape, Walter J. McConathy, Andras G. Lacko

Research output: Contribution to journalArticlepeer-review

95 Scopus citations

Abstract

The purpose of these studies was to determine the mechanism(s) whereby paclitaxel (PTX), is taken up by cancer cells, once encapsulated into synthetic/reconstituted high density lipoprotein (rHDL). The uptake of PTX was found to be facilitated by the scavenger receptor type B-1 (SR-B1) when drug-loaded rHDL particles were incubated with cells that express the SRB1 receptor. Studies with double-labeled, PTX containing rHDL nanoparticles showed that prostate cancer (PC-3) cells incorporated PTX primarily via a selective (SR-B1 type) uptake mechanism. In the presence of a 10-fold excess of plasma HDL, PTX uptake decreased to 30% of the control. These findings suggest that the incorporation of lipophilic drugs by cancer cells from rHDL nanoparticles is facilitated by a receptor mediated (SR-B1) mechanism.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalJournal of Drug Targeting
Volume18
Issue number1
DOIs
StatePublished - 1 Jan 2010

Keywords

  • Folate receptor
  • Lipid nanoparticles
  • Lipoprotein
  • Paclitaxel
  • Receptor uptake
  • Targeted drug delivery

Fingerprint Dive into the research topics of 'Receptor mediated uptake of paclitaxel from a synthetic high density lipoprotein nanocarrier'. Together they form a unique fingerprint.

Cite this