Rational Engineering of CRISPR-Cas9 Nuclease to Attenuate Position-Dependent Off-Target Effects

Keyphrases

• Cas9 Protein 100%
• Rational Engineering 100%
• Off-target Effects 100%
• Position-dependent 100%
• Cas9 nuclease 100%
• Cas9 Variants 66%
• Enhanced Specificity 66%
• Human Cells 33%
• Genome Editing 33%
• Amino Acid Substitution 33%
• Molecular Dynamics Simulation 33%
• Target DNA 33%
• High Specificity 33%
• Biomedical Applications 33%
• Multiple Domains 33%
• Streptococcus Pyogenes (S. pyogenes) 33%
• DNA Cleavage 33%
• RNA-based 33%
• DNA Editing 33%
• Structural Information 33%
• Solid Foundation 33%
• Gene Editing 33%
• Complex Structure 33%
• In Vitro Cleavage 33%
• Atomic Model 33%
• Engineering Strategies 33%
• Single Domain 33%
• Cleavage Assay 33%
• Dynamic Information 33%

Immunology and Microbiology

• CRISPR/Cas9 100%
• Gene Editing 100%
• Nuclease 100%
• Wild Type 50%
• Human Cell 50%
• Dynamics 50%
• Streptococcus pyogenes 50%
• DNA Cleavage 50%
• Amino Acid Substitution 50%

Biochemistry, Genetics and Molecular Biology

• Nuclease 100%
• Cas9 100%
• CRISPR/Cas9 100%
• Enzyme 28%
• Genome Editing 28%
• Wild Type 14%
• RNA 14%
• Dynamics 14%
• DNA Cleavage 14%
• Streptococcus pyogenes 14%
• Amino Acid Substitution 14%