Rapid repeatable in vivo detection of retinal reactive oxygen species

Ning Fan, Sean M. Silverman, Yang Liu, Xizhen Wang, Byung Jin Kim, Liping Tang, Abbot F. Clark, Xuyang Liu, Iok Hou Pang

Research output: Contribution to journalShort survey

2 Citations (Scopus)

Abstract

Oxidative injuries, such as those related to reactive oxygen species (ROS), have been implicated in various retinal and optic nerve disorders. Many ROS detection methods have been developed. Although widely utilized, many of these methods are useful only in post mortem tissues, or require relatively expensive equipment, or involve intraocular injection. In the present study, we demonstrated and characterized a chemiluminescent probe L-012 as a noninvasive, in vivo ROS detection agent in the mouse retina. Using optic nerve crush (ONC) and retinal ischemia/reperfusion (I/R) as injury models, we show that L-012 produced intensive luminescent signals specifically in the injured eyes. Histological examination showed that L-012 administration was safe to the retina. Additionally, compounds that reduce tissue superoxide levels, apocynin and TEMPOL, decreased injury-induced L-012 chemiluminescence. The decrease in L-012 signals correlated with their protective effects against retinal I/R-induced morphological and functional changes in the retina. Together, these data demonstrate the feasibility of a fast, simple, reproducible, and non-invasive detection method to monitor in vivo ROS in the retina. Furthermore, the results also show that reduction of ROS is a potential therapeutic approach for protection from these retinal injuries.

Original languageEnglish
Pages (from-to)71-81
Number of pages11
JournalExperimental eye research
Volume161
DOIs
StatePublished - Aug 2017

Fingerprint

L 012
Reactive Oxygen Species
Retina
Optic Nerve
Wounds and Injuries
Intraocular Injections
Nerve Crush
Luminescence
Reperfusion Injury
Superoxides
Reperfusion
Ischemia
Equipment and Supplies

Keywords

  • Ischemia
  • L-012
  • Mouse
  • Optic nerve injury
  • Oxidative stress
  • Reactive oxidative species
  • Reperfusion
  • Retina

Cite this

Fan, Ning ; Silverman, Sean M. ; Liu, Yang ; Wang, Xizhen ; Kim, Byung Jin ; Tang, Liping ; Clark, Abbot F. ; Liu, Xuyang ; Pang, Iok Hou. / Rapid repeatable in vivo detection of retinal reactive oxygen species. In: Experimental eye research. 2017 ; Vol. 161. pp. 71-81.
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abstract = "Oxidative injuries, such as those related to reactive oxygen species (ROS), have been implicated in various retinal and optic nerve disorders. Many ROS detection methods have been developed. Although widely utilized, many of these methods are useful only in post mortem tissues, or require relatively expensive equipment, or involve intraocular injection. In the present study, we demonstrated and characterized a chemiluminescent probe L-012 as a noninvasive, in vivo ROS detection agent in the mouse retina. Using optic nerve crush (ONC) and retinal ischemia/reperfusion (I/R) as injury models, we show that L-012 produced intensive luminescent signals specifically in the injured eyes. Histological examination showed that L-012 administration was safe to the retina. Additionally, compounds that reduce tissue superoxide levels, apocynin and TEMPOL, decreased injury-induced L-012 chemiluminescence. The decrease in L-012 signals correlated with their protective effects against retinal I/R-induced morphological and functional changes in the retina. Together, these data demonstrate the feasibility of a fast, simple, reproducible, and non-invasive detection method to monitor in vivo ROS in the retina. Furthermore, the results also show that reduction of ROS is a potential therapeutic approach for protection from these retinal injuries.",
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Rapid repeatable in vivo detection of retinal reactive oxygen species. / Fan, Ning; Silverman, Sean M.; Liu, Yang; Wang, Xizhen; Kim, Byung Jin; Tang, Liping; Clark, Abbot F.; Liu, Xuyang; Pang, Iok Hou.

In: Experimental eye research, Vol. 161, 08.2017, p. 71-81.

Research output: Contribution to journalShort survey

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AU - Clark, Abbot F.

AU - Liu, Xuyang

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