Radiative decay engineering: 2. Effects of silver island films on fluorescence intensity, lifetimes, and resonance energy transfer

Joseph R. Lakowicz, Yibing Shen, Sabato D'Auria, Joanna Malicka, Jiyu Fang, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journalArticle

556 Citations (Scopus)

Abstract

Metallic surfaces can have unusual effects on fluorophores such as increasing or decreasing the rates of radiative decay and the rates of resonance energy transfer (RET). In the present article we describe the effects of metallic silver island films on the emission spectra, lifetimes, and energy transfer for several fluorophores. The fluorophores are not covalently coupled to the silver islands so that there are a range of fluorophore-to-metal distances. We show that proximity of fluorophores to the silver islands results in increased fluorescence intensity, with the largest enhancement for the lowest-quantum-yield fluorophores. Importantly, the metal-induced increases in intensity are accompanied by decreased lifetimes and increased photostability. These effects demonstrate that the silver islands have increased the radiative decay rates of the fluorophore. For solvent-sensitive fluorophores the emission spectra shifted to shorted wavelengths in the presence of the silver islands, which is consistent with a decrease of the apparent lifetime for fluorophores near the metal islands. We also observed an increased intensity and blue spectral shift for the protein human glyoxalase, which displays a low quantum yield for its intrinsic tryptophan emission. In this case the blue shift is thought to be due to increased emission from a buried low-quantum-yield tryptophan residue. Increased intensities were also observed for the intrinsic emission of the nucleic acid bases adenine and thymine and for single-stranded 15-mers poly(T) and poly(C). And finally, we observed increased RET for donors and acceptors in solution and when bound to double-helical DNA. These results demonstrate that metallic particles can be used to modify the emission from intrinsic and extrinsic fluorophores in biochemical systems.

Original languageEnglish
Pages (from-to)261-277
Number of pages17
JournalAnalytical Biochemistry
Volume301
Issue number2
DOIs
StatePublished - 15 Feb 2002

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Fluorophores
Energy Transfer
Silver
Islands
Energy transfer
Fluorescence
Metals
Tryptophan
Quantum yield
Poly T
Thymine
Adenine
Nucleic Acids
DNA
Proteins
Wavelength

Cite this

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title = "Radiative decay engineering: 2. Effects of silver island films on fluorescence intensity, lifetimes, and resonance energy transfer",
abstract = "Metallic surfaces can have unusual effects on fluorophores such as increasing or decreasing the rates of radiative decay and the rates of resonance energy transfer (RET). In the present article we describe the effects of metallic silver island films on the emission spectra, lifetimes, and energy transfer for several fluorophores. The fluorophores are not covalently coupled to the silver islands so that there are a range of fluorophore-to-metal distances. We show that proximity of fluorophores to the silver islands results in increased fluorescence intensity, with the largest enhancement for the lowest-quantum-yield fluorophores. Importantly, the metal-induced increases in intensity are accompanied by decreased lifetimes and increased photostability. These effects demonstrate that the silver islands have increased the radiative decay rates of the fluorophore. For solvent-sensitive fluorophores the emission spectra shifted to shorted wavelengths in the presence of the silver islands, which is consistent with a decrease of the apparent lifetime for fluorophores near the metal islands. We also observed an increased intensity and blue spectral shift for the protein human glyoxalase, which displays a low quantum yield for its intrinsic tryptophan emission. In this case the blue shift is thought to be due to increased emission from a buried low-quantum-yield tryptophan residue. Increased intensities were also observed for the intrinsic emission of the nucleic acid bases adenine and thymine and for single-stranded 15-mers poly(T) and poly(C). And finally, we observed increased RET for donors and acceptors in solution and when bound to double-helical DNA. These results demonstrate that metallic particles can be used to modify the emission from intrinsic and extrinsic fluorophores in biochemical systems.",
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Radiative decay engineering : 2. Effects of silver island films on fluorescence intensity, lifetimes, and resonance energy transfer. / Lakowicz, Joseph R.; Shen, Yibing; D'Auria, Sabato; Malicka, Joanna; Fang, Jiyu; Gryczynski, Zygmunt; Gryczynski, Ignacy.

In: Analytical Biochemistry, Vol. 301, No. 2, 15.02.2002, p. 261-277.

Research output: Contribution to journalArticle

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T1 - Radiative decay engineering

T2 - 2. Effects of silver island films on fluorescence intensity, lifetimes, and resonance energy transfer

AU - Lakowicz, Joseph R.

AU - Shen, Yibing

AU - D'Auria, Sabato

AU - Malicka, Joanna

AU - Fang, Jiyu

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

PY - 2002/2/15

Y1 - 2002/2/15

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