QUENCHING OF METHYLCYCLOHEXANE FLUORESCENCE BY METHANOL

Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We measured the fluorescence emission spectrum and intensity decays of methylcyclohexane (MCH) when excited by simultaneous absorption of two photons at 298 nm. The steady‐state intensities and lifetimes were both decreased by methanol, which was found to be an efficient quencher of MCH fluorescence. Methanol quenching of MCH is clearly dynamic, but the exact mechanism of quenching is unclear. Dynamic quenching of MCH was also observed by water and n‐propanol. These results suggest that alkane fluorescence from biopolymers, if observable, will only occur from regions of the macromolecules that are not exposed to water.

Original languageEnglish
Pages (from-to)426-432
Number of pages7
JournalPhotochemistry and Photobiology
Volume62
Issue number3
DOIs
StatePublished - Sep 1995

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