Quantification of mtDNA mixtures in forensic evidence material using pyrosequencing

H. Andréasson, M. Nilsson, Bruce Budowle, S. Frisk, M. Allen

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Analysis of mtDNA variation using Sanger sequencing does not allow accurate quantification of the components of mtDNA mixtures. An alternative method to determine the specific mixture ratios in samples displaying heteroplasmy, consisting of DNA contributions from several individuals, or containing contamination would therefore be valuable. A novel quantification system for mtDNA mixture analysis has been developed based on pyrosequencing technology, in which the linear relationship between incorporated nucleotides and released light allows quantification of the components of a sample. Within five polymerase chain reaction fragments, seven variable positions in the mtDNA control and coding region were evaluated using this quantification analysis. For all single nucleotide polymorphisms quantified in this study, a linear relationship was observed between the measured and expected mixture ratios. This mtDNA quantification assay is an easy to use, fast and accurate quantification system, with the ability to resolve and interpret major and minor mtDNA components in forensic mixture samples.

Original languageEnglish
Pages (from-to)383-390
Number of pages8
JournalInternational Journal of Legal Medicine
Volume120
Issue number6
DOIs
StatePublished - 1 Nov 2006

Keywords

  • Forensic material
  • Mitochondrial DNA
  • Mixtures
  • Pyrosequencing
  • Quantification

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