Quantification of bone changes in a collagen-induced arthritis mouse model by reconstructed three dimensional micro-CT

Shu Yang, Anne M. Hollister, Elysse A. Orchard, Shubnum I. Chaudhery, Dmitry V. Ostanin, Stephen J. Lokitz, James Michael Mathis

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)

Abstract

Background: Inflammatory arthritis is a chronic disease, resulting in synovitis and subchondral and bone area destruction, which can severely affect a patient's quality of life. The most common form of inflammatory arthritis is rheumatoid arthritis (RA) in which many of the disease mechanisms are not well understood. The collagen-induced arthritis (CIA) mouse model is similar to RA as it exhibits joint space narrowing and bone erosion as well as involves inflammatory factors and cellular players that have been implicated in RA pathogenesis. Quantitative data for disease progression in RA models is difficult to obtain as serum blood markers may not always reflect disease state and physical disease indexes are subjective. Thus, it is important to develop tools to objectively assess disease progression in CIA. Results: Micro-CT (Computed Tomography) is a relatively mature technology that has been used to track a variety of anatomical changes in small animals. In this study, micro-CT scans of several joints of control and CIA mice were acquired at 0, 4, 7, and 9 weeks after the immunization with collagen type II. Each micro-CT scan was analyzed by applying a segmentation algorithm to individual slices in each image set to provide 3-dimensional representations of specific bones including the humerus, femur, and tibia. From these representations, the volume and mean density of these bones were measured and compared. This analysis showed that both the volume and the density of each measured bone of the CIA mice were significantly smaller than those of the controls at week 7. Conclusions: This study demonstrates that micro-CT can be used to quantify bone changes in the CIA mouse model as an alternative to disease index assessments. In conclusion, micro-CT could be useful as a non-invasive method to monitor the efficacy of new treatments for RA tested in small animals.

Original languageEnglish
Article number8
JournalBiological Procedures Online
Volume15
Issue number1
DOIs
StatePublished - 18 Jul 2013

Fingerprint

Experimental Arthritis
Rheumatoid Arthritis
Bone
Collagen
Bone and Bones
Arthritis
Disease Progression
Computerized tomography
Joints
Synovitis
Collagen Type II
Animals
Humerus
Tibia
Immunization
Bone Density
Femur
Chronic Disease
Biomarkers
Tomography

Keywords

  • Bone density
  • Bone volume
  • Collagen induced arthritis
  • Computed tomography
  • Disease index
  • Imaging
  • Micro-CT
  • Rheumatoid arthritis

Cite this

Yang, Shu ; Hollister, Anne M. ; Orchard, Elysse A. ; Chaudhery, Shubnum I. ; Ostanin, Dmitry V. ; Lokitz, Stephen J. ; Mathis, James Michael. / Quantification of bone changes in a collagen-induced arthritis mouse model by reconstructed three dimensional micro-CT. In: Biological Procedures Online. 2013 ; Vol. 15, No. 1.
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abstract = "Background: Inflammatory arthritis is a chronic disease, resulting in synovitis and subchondral and bone area destruction, which can severely affect a patient's quality of life. The most common form of inflammatory arthritis is rheumatoid arthritis (RA) in which many of the disease mechanisms are not well understood. The collagen-induced arthritis (CIA) mouse model is similar to RA as it exhibits joint space narrowing and bone erosion as well as involves inflammatory factors and cellular players that have been implicated in RA pathogenesis. Quantitative data for disease progression in RA models is difficult to obtain as serum blood markers may not always reflect disease state and physical disease indexes are subjective. Thus, it is important to develop tools to objectively assess disease progression in CIA. Results: Micro-CT (Computed Tomography) is a relatively mature technology that has been used to track a variety of anatomical changes in small animals. In this study, micro-CT scans of several joints of control and CIA mice were acquired at 0, 4, 7, and 9 weeks after the immunization with collagen type II. Each micro-CT scan was analyzed by applying a segmentation algorithm to individual slices in each image set to provide 3-dimensional representations of specific bones including the humerus, femur, and tibia. From these representations, the volume and mean density of these bones were measured and compared. This analysis showed that both the volume and the density of each measured bone of the CIA mice were significantly smaller than those of the controls at week 7. Conclusions: This study demonstrates that micro-CT can be used to quantify bone changes in the CIA mouse model as an alternative to disease index assessments. In conclusion, micro-CT could be useful as a non-invasive method to monitor the efficacy of new treatments for RA tested in small animals.",
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Quantification of bone changes in a collagen-induced arthritis mouse model by reconstructed three dimensional micro-CT. / Yang, Shu; Hollister, Anne M.; Orchard, Elysse A.; Chaudhery, Shubnum I.; Ostanin, Dmitry V.; Lokitz, Stephen J.; Mathis, James Michael.

In: Biological Procedures Online, Vol. 15, No. 1, 8, 18.07.2013.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Quantification of bone changes in a collagen-induced arthritis mouse model by reconstructed three dimensional micro-CT

AU - Yang, Shu

AU - Hollister, Anne M.

AU - Orchard, Elysse A.

AU - Chaudhery, Shubnum I.

AU - Ostanin, Dmitry V.

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AU - Mathis, James Michael

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AB - Background: Inflammatory arthritis is a chronic disease, resulting in synovitis and subchondral and bone area destruction, which can severely affect a patient's quality of life. The most common form of inflammatory arthritis is rheumatoid arthritis (RA) in which many of the disease mechanisms are not well understood. The collagen-induced arthritis (CIA) mouse model is similar to RA as it exhibits joint space narrowing and bone erosion as well as involves inflammatory factors and cellular players that have been implicated in RA pathogenesis. Quantitative data for disease progression in RA models is difficult to obtain as serum blood markers may not always reflect disease state and physical disease indexes are subjective. Thus, it is important to develop tools to objectively assess disease progression in CIA. Results: Micro-CT (Computed Tomography) is a relatively mature technology that has been used to track a variety of anatomical changes in small animals. In this study, micro-CT scans of several joints of control and CIA mice were acquired at 0, 4, 7, and 9 weeks after the immunization with collagen type II. Each micro-CT scan was analyzed by applying a segmentation algorithm to individual slices in each image set to provide 3-dimensional representations of specific bones including the humerus, femur, and tibia. From these representations, the volume and mean density of these bones were measured and compared. This analysis showed that both the volume and the density of each measured bone of the CIA mice were significantly smaller than those of the controls at week 7. Conclusions: This study demonstrates that micro-CT can be used to quantify bone changes in the CIA mouse model as an alternative to disease index assessments. In conclusion, micro-CT could be useful as a non-invasive method to monitor the efficacy of new treatments for RA tested in small animals.

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