Pyruvate-enriched resuscitation: Metabolic support of post-ischemic hindlimb muscle in hypovolemic goats

Hunaid A. Gurji, Daniel W. White, Besim Hoxha, Jie Sun, Jessica P. Harbor, Diana R. Schulz, Arthur G. Williams, Albert H. Olivencia-Yurvati, Robert T. Mallet

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Tourniquet-imposed ischemia-reperfusion of extremities generates reactive oxygen and nitrogen species (RONS), which can disrupt intermediary metabolism and ATP production. This study tested the hypothesis that fluid resuscitation with pyruvate, a natural antioxidant and metabolic fuel, ameliorates the deleterious effects of ischemia-reperfusion on intermediary metabolism in skeletal muscle. Anesthetized male goats (~25 kg) were bled to a mean arterial pressure of 48 ± 1 mmHg and then subjected to 90 min hindlimb ischemia with a tourniquet and femoral crossclamp, followed by 4-h reperfusion. Lactated Ringers (LR) or pyruvate Ringers (PR) was infused intravenous for 90 min, from 30 min ischemia to 30 min reperfusion, to deliver 0.05 mmol kg-1 min-1 lactate or pyruvate. Time controls (TC) underwent neither hemorrhage nor hindlimb ischemia. Lipid peroxidation product 8-isoprostane, RONS-sensitive aconitase and creatine kinase activities, antioxidant superoxide dismutase activity, and phosphocreatine phosphorylation potential ([PCr]/[{Cr}{Pi}]), an index of tissue energy state, were measured in reperfused gastrocnemius at 90 min resuscitation (n = 6 all groups) and 3.5 h post-resuscitation (n = 8 TC, 9 LR, 10 PR). PR more effectively than LR suppressed 8-isoprostane formation, prevented inactivation of aconitase and creatine kinase, doubled superoxide dismutase activity, and augmented [PCr]/([Cr][Pi]). Pyruvate-enriched Ringer's is metabolically superior to Ringer's lactate for fluid resuscitation of tourniqueted muscle.

Original languageEnglish
Pages (from-to)240-249
Number of pages10
JournalExperimental Biology and Medicine
Volume239
Issue number2
DOIs
StatePublished - 1 Feb 2014

Fingerprint

Resuscitation
Hypovolemia
Hindlimb
Pyruvic Acid
Goats
Muscle
8-epi-prostaglandin F2alpha
Ischemia
Muscles
Reperfusion
Aconitate Hydratase
Reactive Nitrogen Species
Tourniquets
Creatine Kinase
Metabolism
Superoxide Dismutase
Reactive Oxygen Species
Antioxidants
Phosphorylation
Fluids

Keywords

  • 8-isoprostane
  • Creatine kinase
  • hypovolemia
  • phosphorylation potential

Cite this

Gurji, Hunaid A. ; White, Daniel W. ; Hoxha, Besim ; Sun, Jie ; Harbor, Jessica P. ; Schulz, Diana R. ; Williams, Arthur G. ; Olivencia-Yurvati, Albert H. ; Mallet, Robert T. / Pyruvate-enriched resuscitation : Metabolic support of post-ischemic hindlimb muscle in hypovolemic goats. In: Experimental Biology and Medicine. 2014 ; Vol. 239, No. 2. pp. 240-249.
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Pyruvate-enriched resuscitation : Metabolic support of post-ischemic hindlimb muscle in hypovolemic goats. / Gurji, Hunaid A.; White, Daniel W.; Hoxha, Besim; Sun, Jie; Harbor, Jessica P.; Schulz, Diana R.; Williams, Arthur G.; Olivencia-Yurvati, Albert H.; Mallet, Robert T.

In: Experimental Biology and Medicine, Vol. 239, No. 2, 01.02.2014, p. 240-249.

Research output: Contribution to journalArticle

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AU - Gurji, Hunaid A.

AU - White, Daniel W.

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AU - Harbor, Jessica P.

AU - Schulz, Diana R.

AU - Williams, Arthur G.

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