Pyruvate-enriched resuscitation

Metabolic support of post-ischemic hindlimb muscle in hypovolemic goats

Hunaid A. Gurji, Daniel W. White, Besim Hoxha, Jie Sun, Jessica P. Harbor, Diana R. Schulz, Arthur G. Williams, Albert Yurvati, Robert T. Mallet

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

Tourniquet-imposed ischemia-reperfusion of extremities generates reactive oxygen and nitrogen species (RONS), which can disrupt intermediary metabolism and ATP production. This study tested the hypothesis that fluid resuscitation with pyruvate, a natural antioxidant and metabolic fuel, ameliorates the deleterious effects of ischemia-reperfusion on intermediary metabolism in skeletal muscle. Anesthetized male goats (~25 kg) were bled to a mean arterial pressure of 48 ± 1 mmHg and then subjected to 90 min hindlimb ischemia with a tourniquet and femoral crossclamp, followed by 4-h reperfusion. Lactated Ringers (LR) or pyruvate Ringers (PR) was infused intravenous for 90 min, from 30 min ischemia to 30 min reperfusion, to deliver 0.05 mmol kg-1 min-1 lactate or pyruvate. Time controls (TC) underwent neither hemorrhage nor hindlimb ischemia. Lipid peroxidation product 8-isoprostane, RONS-sensitive aconitase and creatine kinase activities, antioxidant superoxide dismutase activity, and phosphocreatine phosphorylation potential ([PCr]/[{Cr}{Pi}]), an index of tissue energy state, were measured in reperfused gastrocnemius at 90 min resuscitation (n = 6 all groups) and 3.5 h post-resuscitation (n = 8 TC, 9 LR, 10 PR). PR more effectively than LR suppressed 8-isoprostane formation, prevented inactivation of aconitase and creatine kinase, doubled superoxide dismutase activity, and augmented [PCr]/([Cr][Pi]). Pyruvate-enriched Ringer's is metabolically superior to Ringer's lactate for fluid resuscitation of tourniqueted muscle.

Original languageEnglish
Pages (from-to)240-249
Number of pages10
JournalExperimental Biology and Medicine
Volume239
Issue number2
DOIs
StatePublished - 1 Feb 2014

Fingerprint

Resuscitation
Hypovolemia
Hindlimb
Pyruvic Acid
Goats
Muscle
8-epi-prostaglandin F2alpha
Ischemia
Muscles
Reperfusion
Aconitate Hydratase
Reactive Nitrogen Species
Tourniquets
Creatine Kinase
Metabolism
Superoxide Dismutase
Reactive Oxygen Species
Antioxidants
Phosphorylation
Fluids

Keywords

  • 8-isoprostane
  • Creatine kinase
  • hypovolemia
  • phosphorylation potential

Cite this

Gurji, Hunaid A. ; White, Daniel W. ; Hoxha, Besim ; Sun, Jie ; Harbor, Jessica P. ; Schulz, Diana R. ; Williams, Arthur G. ; Yurvati, Albert ; Mallet, Robert T. / Pyruvate-enriched resuscitation : Metabolic support of post-ischemic hindlimb muscle in hypovolemic goats. In: Experimental Biology and Medicine. 2014 ; Vol. 239, No. 2. pp. 240-249.
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Pyruvate-enriched resuscitation : Metabolic support of post-ischemic hindlimb muscle in hypovolemic goats. / Gurji, Hunaid A.; White, Daniel W.; Hoxha, Besim; Sun, Jie; Harbor, Jessica P.; Schulz, Diana R.; Williams, Arthur G.; Yurvati, Albert; Mallet, Robert T.

In: Experimental Biology and Medicine, Vol. 239, No. 2, 01.02.2014, p. 240-249.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Gurji, Hunaid A.

AU - White, Daniel W.

AU - Hoxha, Besim

AU - Sun, Jie

AU - Harbor, Jessica P.

AU - Schulz, Diana R.

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KW - 8-isoprostane

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KW - phosphorylation potential

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