TY - JOUR
T1 - Prototype PowerPlex® Y23 System
T2 - A concordance study
AU - Davis, Carey
AU - Ge, Jianye
AU - Sprecher, Cynthia
AU - Chidambaram, Abirami
AU - Thompson, Jonelle
AU - Ewing, Margaret
AU - Fulmer, Patricia
AU - Rabbach, Dawn
AU - Storts, Douglas
AU - Budowle, Bruce
PY - 2013/1
Y1 - 2013/1
N2 - The Prototype PowerPlex® Y23 System (Promega Corporation, Madison, WI) is a polymerase chain reaction-based amplification kit that targets the 23 Y STR loci DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, and Y-GATA-H4. A total of 951 samples from six populations were typed to evaluate the kit and examine concordance for 17 of the loci that are in common with those that can be typed using the AmpFlSTR ® Yfiler™ kit (Life Technologies, Carlsbad, CA). A total of 16,167 loci were analyzed for each multiplex, and overall concordance was observed. Because of different kit designs, and although concordant for the genetic type, discordant calls can occur due to a deletion at the DYS448 locus. Users should take into consideration such nomenclature anomalies when comparing Y STR profiles. This new kit allows a large battery of Y STR loci to be analyzed using the same basic technologies already employed in forensic laboratories.
AB - The Prototype PowerPlex® Y23 System (Promega Corporation, Madison, WI) is a polymerase chain reaction-based amplification kit that targets the 23 Y STR loci DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, and Y-GATA-H4. A total of 951 samples from six populations were typed to evaluate the kit and examine concordance for 17 of the loci that are in common with those that can be typed using the AmpFlSTR ® Yfiler™ kit (Life Technologies, Carlsbad, CA). A total of 16,167 loci were analyzed for each multiplex, and overall concordance was observed. Because of different kit designs, and although concordant for the genetic type, discordant calls can occur due to a deletion at the DYS448 locus. Users should take into consideration such nomenclature anomalies when comparing Y STR profiles. This new kit allows a large battery of Y STR loci to be analyzed using the same basic technologies already employed in forensic laboratories.
KW - Forensic DNA analysis
KW - Native Alaskans
KW - STR
KW - US populations
KW - Y chromosome
KW - concordance
UR - http://www.scopus.com/inward/record.url?scp=84869499738&partnerID=8YFLogxK
U2 - 10.1016/j.fsigen.2012.06.005
DO - 10.1016/j.fsigen.2012.06.005
M3 - Article
C2 - 22749790
AN - SCOPUS:84869499738
SN - 1872-4973
VL - 7
SP - 204
EP - 208
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
IS - 1
ER -