Abstract
We used high-throughput Western blotting to identify proteins that are up- or down-regulated by neuronal hypoxia in vitro. Exposure to hypoxia for 24 h produced ≥ 1.5-fold increases in the expression of 10/700 proteins (1.4%) and decreases in the expression of 16/700 proteins (2.3%). Up-regulated proteins included Arc, doublecortin/calmodulin kinase-like 1, integrin αv, and fibronectin; down-regulated proteins included nuclear autoantigenic sperm protein, protein kinase C-related kinase 2, and E2F transcription factor 1. The prominence of cytoskeleton-related proteins among those showing altered expression highlights the role of the cytoskeleton in neuronal responses to hypoxia.
Original language | English |
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Pages (from-to) | 1123-1128 |
Number of pages | 6 |
Journal | Neurochemical Research |
Volume | 29 |
Issue number | 6 |
DOIs | |
State | Published - 1 Jun 2004 |
Keywords
- Activity-regulated cytoskeleton-associated protein
- Cytoskeleton
- Hypoxia
- Neurogenesis
- Neuron
- Proteomics