Proteomic analysis of neuronal hypoxia in vitro

K. Jin, X. O. Mao, D. A. Greenberg

Research output: Contribution to journalReview article

19 Scopus citations

Abstract

We used high-throughput Western blotting to identify proteins that are up- or down-regulated by neuronal hypoxia in vitro. Exposure to hypoxia for 24 h produced ≥ 1.5-fold increases in the expression of 10/700 proteins (1.4%) and decreases in the expression of 16/700 proteins (2.3%). Up-regulated proteins included Arc, doublecortin/calmodulin kinase-like 1, integrin αv, and fibronectin; down-regulated proteins included nuclear autoantigenic sperm protein, protein kinase C-related kinase 2, and E2F transcription factor 1. The prominence of cytoskeleton-related proteins among those showing altered expression highlights the role of the cytoskeleton in neuronal responses to hypoxia.

Original languageEnglish
Pages (from-to)1123-1128
Number of pages6
JournalNeurochemical Research
Volume29
Issue number6
DOIs
StatePublished - 1 Jun 2004

Keywords

  • Activity-regulated cytoskeleton-associated protein
  • Cytoskeleton
  • Hypoxia
  • Neurogenesis
  • Neuron
  • Proteomics

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