Proteomic Analysis of Mouse Brain Microsomes: Identification and Bioinformatic Characterization of Endoplasmic Reticulum Proteins in the Mammalian Central Nervous System

Stanley M. Stevens, R. Scott Duncan, Peter Koulen, Laszlo Prokai

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The endoplasmic reticulum (ER) is the main source for the storage and release of intracellular calcium in neurons and, thus, contributes to the functionality of a diverse set of pathways that control critical aspects of central nervous system function including but not limited to gene expression, neurotransmission, learning, and memory. ER-derived proteins obtained after subcellular fractionation of mouse brain homogenate were digested with trypsin and the corresponding peptides fractionated by strong cation exchange chromatography followed by LC-MS/MS analysis on a hybrid linear ion trap-Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. A comprehensive catalogue representing 1914 proteins was generated from this particular proteomic analysis using identification criteria that corresponded to a false positive identification rate of 0.4%. Various molecular functions and biological processes relevant to the ER were identified upon gene ontology (GO)-based analysis including pathways associated with molecular transport, protein trafficking and localization, and cell signaling. Comparison of the 2D-LC-MS/MS results with those obtained from shotgun LC-MS/MS analyses demonstrated that most molecular functions and biological processes were represented via GO analysis using either methodology. Results from this comparison as well as a focused investigation into components of calcium-mediated signaling in the mouse brain ER are also presented.

Original languageEnglish
Pages (from-to)1046-1054
Number of pages9
JournalJournal of Proteome Research
Volume7
Issue number3
DOIs
StatePublished - 1 Mar 2008

Keywords

  • Brain microsomes
  • Calcium signaling
  • Endoplasmic
  • Fractionation, fourier transform ion cyclotron resonance mass spectrometry
  • Proteomics
  • Reticulum
  • Subcellular

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