Protein Profiling of human nonpigmented ciliary epithelium cell secretome

The differentiation factors characterization for retinal ganglion cell line

Tze Wen Chung, Ming Hui Yang, Raghu Krishnamoorthy, Shiang Bin Jong, Pei Yu Chu, Yuan Han Yang, Wen Cheng Chen, Sharon Chia Ju Chen, Adnan Dibas, Thomas Yorio, Yu Chang Tyan

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

The purpose of this paper was to characterize proteins secreted from the human nonpigmented ciliary epithelial (HNPE) cells, which have differentiated a rat retinal ganglion cell line, RGC-5. Undifferentiated RGC-5 cells have been shown to express several marker proteins characteristic of retinal ganglion cells. However, RGC-5 cells do not respond to N-methyl-D aspartate (NMDA), or glutamate. HNPE cells have been shown to secrete numbers of neuropeptides or neuroproteins also found in the aqueous humor, many of which have the ability to influence the activity of neuronal cells. This paper details the profile of HNPE cell-secreted proteins by proteomic approaches. The experimental results revealed the identification of 132 unique proteins from the HNPE cell-conditioned SF-medium. The biological functions of a portion of these identified proteins are involved in cell differentiation. We hypothesized that a differentiation system of HNPE cell-conditioned SF-medium with RGC-5 cells can induce a differentiated phenotype in RGC-5 cells, with functional characteristics that more closely resemble primary cultures of rat retinal ganglion cells. These proteins may replace harsh chemicals, which are currently used to induce cell differentiation.

Original languageEnglish
Article number901329
JournalJournal of Biomedicine and Biotechnology
Volume2011
DOIs
StatePublished - 12 Oct 2011

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Retinal Ganglion Cells
Epithelium
Cell Line
Epithelial Cells
Proteins
Conditioned Culture Medium
Cell Differentiation
Aqueous Humor
N-Methylaspartate
Neuropeptides
Proteomics
Phenotype

Cite this

Chung, Tze Wen ; Yang, Ming Hui ; Krishnamoorthy, Raghu ; Jong, Shiang Bin ; Chu, Pei Yu ; Yang, Yuan Han ; Chen, Wen Cheng ; Chen, Sharon Chia Ju ; Dibas, Adnan ; Yorio, Thomas ; Tyan, Yu Chang. / Protein Profiling of human nonpigmented ciliary epithelium cell secretome : The differentiation factors characterization for retinal ganglion cell line. In: Journal of Biomedicine and Biotechnology. 2011 ; Vol. 2011.
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abstract = "The purpose of this paper was to characterize proteins secreted from the human nonpigmented ciliary epithelial (HNPE) cells, which have differentiated a rat retinal ganglion cell line, RGC-5. Undifferentiated RGC-5 cells have been shown to express several marker proteins characteristic of retinal ganglion cells. However, RGC-5 cells do not respond to N-methyl-D aspartate (NMDA), or glutamate. HNPE cells have been shown to secrete numbers of neuropeptides or neuroproteins also found in the aqueous humor, many of which have the ability to influence the activity of neuronal cells. This paper details the profile of HNPE cell-secreted proteins by proteomic approaches. The experimental results revealed the identification of 132 unique proteins from the HNPE cell-conditioned SF-medium. The biological functions of a portion of these identified proteins are involved in cell differentiation. We hypothesized that a differentiation system of HNPE cell-conditioned SF-medium with RGC-5 cells can induce a differentiated phenotype in RGC-5 cells, with functional characteristics that more closely resemble primary cultures of rat retinal ganglion cells. These proteins may replace harsh chemicals, which are currently used to induce cell differentiation.",
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Protein Profiling of human nonpigmented ciliary epithelium cell secretome : The differentiation factors characterization for retinal ganglion cell line. / Chung, Tze Wen; Yang, Ming Hui; Krishnamoorthy, Raghu; Jong, Shiang Bin; Chu, Pei Yu; Yang, Yuan Han; Chen, Wen Cheng; Chen, Sharon Chia Ju; Dibas, Adnan; Yorio, Thomas; Tyan, Yu Chang.

In: Journal of Biomedicine and Biotechnology, Vol. 2011, 901329, 12.10.2011.

Research output: Contribution to journalArticleResearchpeer-review

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T2 - The differentiation factors characterization for retinal ganglion cell line

AU - Chung, Tze Wen

AU - Yang, Ming Hui

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AU - Jong, Shiang Bin

AU - Chu, Pei Yu

AU - Yang, Yuan Han

AU - Chen, Wen Cheng

AU - Chen, Sharon Chia Ju

AU - Dibas, Adnan

AU - Yorio, Thomas

AU - Tyan, Yu Chang

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N2 - The purpose of this paper was to characterize proteins secreted from the human nonpigmented ciliary epithelial (HNPE) cells, which have differentiated a rat retinal ganglion cell line, RGC-5. Undifferentiated RGC-5 cells have been shown to express several marker proteins characteristic of retinal ganglion cells. However, RGC-5 cells do not respond to N-methyl-D aspartate (NMDA), or glutamate. HNPE cells have been shown to secrete numbers of neuropeptides or neuroproteins also found in the aqueous humor, many of which have the ability to influence the activity of neuronal cells. This paper details the profile of HNPE cell-secreted proteins by proteomic approaches. The experimental results revealed the identification of 132 unique proteins from the HNPE cell-conditioned SF-medium. The biological functions of a portion of these identified proteins are involved in cell differentiation. We hypothesized that a differentiation system of HNPE cell-conditioned SF-medium with RGC-5 cells can induce a differentiated phenotype in RGC-5 cells, with functional characteristics that more closely resemble primary cultures of rat retinal ganglion cells. These proteins may replace harsh chemicals, which are currently used to induce cell differentiation.

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