TY - JOUR
T1 - Protein mass spectrometry detects multiple bloodmeals for enhanced Chagas disease vector ecology
AU - Keller, Judith I.
AU - Lima-Cordón, Raquel
AU - Monroy, M. Carlota
AU - Schmoker, Anna M.
AU - Zhang, Fan
AU - Howard, Alan
AU - Ballif, Bryan A.
AU - Stevens, Lori
N1 - Funding Information:
This work was supported by: NIH grant 8P20GM103449 (BAB) from the INBRE program of the NIGMS, NIH grant R03AI26268/1-2 (LS) from NIAID, NSF grant BCS-1216193 (LS, CM) as part of the joint NSF-NIH-USDA EEID program; IDRC grant 106531 (CM) for insect capture; the University of Vermont CAS Seed grant (LS), University of Vermont CAS Suitor Award (JIK), University of Vermont Graduate College MiniGrant (JIK). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the funders. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank all collaborators at San Carlos University/LENAP for insect sample collection and initial processing, and Kristiaan Finstad and the UVM Stein Lab for providing a mouse blood control sample. Thank you to Jeffrey Knott and Jason Reynolds at Cell Signaling Technology for synthesizing the stable isotope-containing peptides.
Funding Information:
This work was supported by: NIH grant 8P20GM103449 (BAB) from the INBRE program of the NIGMS , NIH grant R03AI26268/1-2 (LS) from NIAID, NSF grant BCS-1216193 (LS, CM) as part of the joint NSF-NIH-USDA EEID program; IDRC grant 106531 (CM) for insect capture; the University of Vermont CAS Seed grant (LS), University of Vermont CAS Suitor Award (JIK), University of Vermont Graduate College MiniGrant (JIK). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the funders. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/10
Y1 - 2019/10
N2 - Chagas disease, a neglected tropical disease endemic in Latin America, is caused by the protozoan parasite Trypanosoma cruzi and is responsible for significant health impacts, especially in rural communities. The parasite is transmitted by insect vectors in the Triatominae subfamily and due to lack of vaccines and limited treatment options, vector control is the main way of controlling the disease. Knowing what vectors are feeding on directly enhances our understanding of the ecology and biology of the different vector species and can potentially aid in engaging communities in active disease control, a concept known as Ecohealth management. We evaluated bloodmeals in rural community, house-caught insect vectors previously evaluated for bloodmeals via DNA analysis as part of a larger collaborative project from three countries in Central America, including Guatemala. In addition to identifying bloodmeals in 100% of all samples using liquid chromatography tandem mass spectrometry (LC-MS/MS) (n = 50), strikingly for 53% of these samples there was no evidence of a recent bloodmeal by DNA-PCR. As individual vectors often feed on multiple sources, we developed an enhanced detection pipeline, and showed the ability to quantify a bloodmeal using stable-isotope-containing synthetic references peptides, a first step in further exploration of species-specific bloodmeal composition. Furthermore, we show that a lower resolution mass spectrometer is sufficient to correctly identify taxa from bloodmeals, an important and strong attribute of our LC-MS/MS-based method, opening the door to using proteomics in countries where Chagas disease is endemic.
AB - Chagas disease, a neglected tropical disease endemic in Latin America, is caused by the protozoan parasite Trypanosoma cruzi and is responsible for significant health impacts, especially in rural communities. The parasite is transmitted by insect vectors in the Triatominae subfamily and due to lack of vaccines and limited treatment options, vector control is the main way of controlling the disease. Knowing what vectors are feeding on directly enhances our understanding of the ecology and biology of the different vector species and can potentially aid in engaging communities in active disease control, a concept known as Ecohealth management. We evaluated bloodmeals in rural community, house-caught insect vectors previously evaluated for bloodmeals via DNA analysis as part of a larger collaborative project from three countries in Central America, including Guatemala. In addition to identifying bloodmeals in 100% of all samples using liquid chromatography tandem mass spectrometry (LC-MS/MS) (n = 50), strikingly for 53% of these samples there was no evidence of a recent bloodmeal by DNA-PCR. As individual vectors often feed on multiple sources, we developed an enhanced detection pipeline, and showed the ability to quantify a bloodmeal using stable-isotope-containing synthetic references peptides, a first step in further exploration of species-specific bloodmeal composition. Furthermore, we show that a lower resolution mass spectrometer is sufficient to correctly identify taxa from bloodmeals, an important and strong attribute of our LC-MS/MS-based method, opening the door to using proteomics in countries where Chagas disease is endemic.
KW - Bloodmeal
KW - Chagas
KW - Ecohealth
KW - LC-MS/MS
KW - Mass spectrometry
KW - Triatoma dimidiata
UR - http://www.scopus.com/inward/record.url?scp=85071321890&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2019.103998
DO - 10.1016/j.meegid.2019.103998
M3 - Article
C2 - 31401306
AN - SCOPUS:85071321890
SN - 1567-1348
VL - 74
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
M1 - 103998
ER -