TY - JOUR
T1 - Protein expression in the Drosophila Schneider 2 cell system.
AU - Schetz, John A.
AU - Shankar, Eswar P.N.
PY - 2004/9
Y1 - 2004/9
N2 - The Schneider-2 (S2) Drosophila cell line is well suited for the stable overexpression of recombinant proteins using plasmid-based protein expression vectors. Following drug selection, a polyclonal S2 cell line can be induced to express on the order of 2 to 100 pmol/mg membrane protein for G-coupled protein receptors, 4000 to 100,000 sites/cell for other membrane receptors and 3 to 35 mg/liter for soluble and secreted proteins.
AB - The Schneider-2 (S2) Drosophila cell line is well suited for the stable overexpression of recombinant proteins using plasmid-based protein expression vectors. Following drug selection, a polyclonal S2 cell line can be induced to express on the order of 2 to 100 pmol/mg membrane protein for G-coupled protein receptors, 4000 to 100,000 sites/cell for other membrane receptors and 3 to 35 mg/liter for soluble and secreted proteins.
UR - http://www.scopus.com/inward/record.url?scp=43149099684&partnerID=8YFLogxK
U2 - 10.1002/0471142301.ns0416s27
DO - 10.1002/0471142301.ns0416s27
M3 - Article
C2 - 18428600
AN - SCOPUS:43149099684
SN - 1934-8576
VL - Chapter 4
SP - Unit 4.16
JO - Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]
JF - Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]
ER -