Protein expression in the Drosophila Schneider 2 cell system.

John A. Schetz, Eswar P.N. Shankar

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The Schneider-2 (S2) Drosophila cell line is well suited for the stable overexpression of recombinant proteins using plasmid-based protein expression vectors. Following drug selection, a polyclonal S2 cell line can be induced to express on the order of 2 to 100 pmol/mg membrane protein for G-coupled protein receptors, 4000 to 100,000 sites/cell for other membrane receptors and 3 to 35 mg/liter for soluble and secreted proteins.

Original languageEnglish
Pages (from-to)Unit 4.16
JournalCurrent protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]
VolumeChapter 4
DOIs
StatePublished - Sep 2004

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