The Schneider-2 (S2) Drosophila cell line is well suited for the stable overexpression of recombinant proteins using plasmid-based protein expression vectors. Following drug selection, a polyclonal S2 cell line can be induced to express on the order of 2 to 100 pmol/mg membrane protein for G-coupled protein receptors, 4000 to 100,000 sites/cell for other membrane receptors and 3 to 35 mg/liter for soluble and secreted proteins.
|Pages (from-to)||Unit 4.16|
|Journal||Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]|
|State||Published - Sep 2004|