Properties of coatings on RFID p-Chips that support plasmonic fluorescence enhancement in bioassays

Ryan Rich, Ji Li, Rafal Fudala, Zygmunt Gryczynski, Ignacy Gryczynski, Wlodek Mandecki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Microtransponders (RFID p-Chips) derivatized with silver island film (SIF) have previously seen success as a platform for the quantification of low-abundance biomolecules in nucleic acid based assays and immunoassays. In this study, we further characterized the morphology of the SIF as well as the polymer matrix enveloping it by scanning electron microscopy (SEM). The polymer was a two-layer silane-based matrix engulfing the p-Chip and SIF. Through a series of SEM and confocal fluorescence microscopy experiments, we found the depth of the polymer matrix to be 1-2 μm. The radiative effects of the SIF/polymer layer were assessed by fluorescence lifetime imaging (FLIM) of p-Chips coated with the polymer to which a fluorophore (Alexa Fluor 555) was conjugated. FLIM images showed an 8.7-fold increase in fluorescence intensity and an increased rate of radiative decay, the latter of which is associated with improved photostability and both of which are linked to plasmonic enhancement by the SIF. Plasmonic enhancement was found to extend uniformly across the p-Chip and, interestingly, to a depth of about 1.2 μm. The substantial depth of enhancement suggests that the SIF/polymer layer constitutes a three-dimensional matrix that is accessible to solvent and small molecules such as fluorescent dyes. Finally, we confirmed that no surface-enhanced Raman scattering is seen from the SIF/polymer combination. The analysis provides a possible mechanism by which the SIF/ polymer-coated p-Chips allow a highly sensitive immunoassay and, as a result, leads to an improved bioassay platform.

Original languageEnglish
Pages (from-to)2223-2231
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume404
Issue number8
DOIs
StatePublished - 1 Nov 2012

Fingerprint

Radio Frequency Identification Device
Bioassay
Silver
Radio frequency identification (RFID)
Islands
Biological Assay
Polymers
Fluorescence
Coatings
Polymer films
Optical Imaging
Polymer matrix
Immunoassay
Electron Scanning Microscopy
Imaging techniques
Silanes
Scanning electron microscopy
Raman Spectrum Analysis
Fluorophores
Confocal microscopy

Keywords

  • ELISA
  • FLIM
  • Multiplex
  • RFID
  • SIF
  • Surface plasmon resonance

Cite this

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title = "Properties of coatings on RFID p-Chips that support plasmonic fluorescence enhancement in bioassays",
abstract = "Microtransponders (RFID p-Chips) derivatized with silver island film (SIF) have previously seen success as a platform for the quantification of low-abundance biomolecules in nucleic acid based assays and immunoassays. In this study, we further characterized the morphology of the SIF as well as the polymer matrix enveloping it by scanning electron microscopy (SEM). The polymer was a two-layer silane-based matrix engulfing the p-Chip and SIF. Through a series of SEM and confocal fluorescence microscopy experiments, we found the depth of the polymer matrix to be 1-2 μm. The radiative effects of the SIF/polymer layer were assessed by fluorescence lifetime imaging (FLIM) of p-Chips coated with the polymer to which a fluorophore (Alexa Fluor 555) was conjugated. FLIM images showed an 8.7-fold increase in fluorescence intensity and an increased rate of radiative decay, the latter of which is associated with improved photostability and both of which are linked to plasmonic enhancement by the SIF. Plasmonic enhancement was found to extend uniformly across the p-Chip and, interestingly, to a depth of about 1.2 μm. The substantial depth of enhancement suggests that the SIF/polymer layer constitutes a three-dimensional matrix that is accessible to solvent and small molecules such as fluorescent dyes. Finally, we confirmed that no surface-enhanced Raman scattering is seen from the SIF/polymer combination. The analysis provides a possible mechanism by which the SIF/ polymer-coated p-Chips allow a highly sensitive immunoassay and, as a result, leads to an improved bioassay platform.",
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Properties of coatings on RFID p-Chips that support plasmonic fluorescence enhancement in bioassays. / Rich, Ryan; Li, Ji; Fudala, Rafal; Gryczynski, Zygmunt; Gryczynski, Ignacy; Mandecki, Wlodek.

In: Analytical and Bioanalytical Chemistry, Vol. 404, No. 8, 01.11.2012, p. 2223-2231.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Properties of coatings on RFID p-Chips that support plasmonic fluorescence enhancement in bioassays

AU - Rich, Ryan

AU - Li, Ji

AU - Fudala, Rafal

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

AU - Mandecki, Wlodek

PY - 2012/11/1

Y1 - 2012/11/1

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KW - FLIM

KW - Multiplex

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