Promotion of incisional wound repair by human mesenchymal stem cell transplantation

Alexander Stoff, Angel A. Rivera, N. Sanjib Banerjee, Steven T. Moore, T. Michael Numnum, Antonio Espinosa-de-los-Monteros, Dirk F. Richter, Gene P. Siegal, Louise T. Chow, Dale Feldman, Luis O. Vasconez, J. Michael Mathis, Mariam A. Stoff-Khalili, David T. Curiel

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to determine the effect of transplanted human mesenchymal stem cells (hMSCs) on wound healing. In this model, full-thickness cutaneous wounds were created by incision in the skin of adult New Zealand white rabbits and treated by transplanted hMSCs into the wounds. Wound healing was evaluated by histological analysis and tensiometry over time. A total of 15 New Zealand white rabbits with 10 wounds per animal were examined in this study. Animals were treated with hMSCs and euthanised at 3, 7, 14, 21 and 80 days after manipulation. The hMSCs were labelled with a fluorescent dye (CM-DiI), suspended in phosphate-buffered saline and used to treat full-thickness incisional wounds in rabbit skin. Tensiometry and histology were used to characterise the wound-healing rate of the incisional wounds. These results showed that transplanted hMSCs significantly inhibited scar formation and increased the tensile strength of the wounds. Importantly, MSCs from genetically unrelated donors did not appear to induce an immunologic response. In conclusion, human mesenchymal stem cell therapy is a viable approach to significantly affect the course of normal cutaneous wound healing and significantly increase the tensile strength.

Original languageEnglish
Pages (from-to)362-369
Number of pages8
JournalExperimental Dermatology
Volume18
Issue number4
DOIs
StatePublished - 1 Apr 2009

Fingerprint

Mesenchymal Stem Cell Transplantation
Stem cells
Mesenchymal Stromal Cells
Repair
Wound Healing
Wounds and Injuries
Skin
Tensile Strength
Rabbits
Animals
Tensile strength
Unrelated Donors
Histology
Cell- and Tissue-Based Therapy
Fluorescent Dyes
Cicatrix
Phosphates

Keywords

  • Incisional wounds
  • Mesenchymal stem cells
  • Scar formation
  • Tensile strength
  • Wound healing

Cite this

Stoff, A., Rivera, A. A., Banerjee, N. S., Moore, S. T., Numnum, T. M., Espinosa-de-los-Monteros, A., ... Curiel, D. T. (2009). Promotion of incisional wound repair by human mesenchymal stem cell transplantation. Experimental Dermatology, 18(4), 362-369. https://doi.org/10.1111/j.1600-0625.2008.00792.x
Stoff, Alexander ; Rivera, Angel A. ; Banerjee, N. Sanjib ; Moore, Steven T. ; Numnum, T. Michael ; Espinosa-de-los-Monteros, Antonio ; Richter, Dirk F. ; Siegal, Gene P. ; Chow, Louise T. ; Feldman, Dale ; Vasconez, Luis O. ; Mathis, J. Michael ; Stoff-Khalili, Mariam A. ; Curiel, David T. / Promotion of incisional wound repair by human mesenchymal stem cell transplantation. In: Experimental Dermatology. 2009 ; Vol. 18, No. 4. pp. 362-369.
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Stoff, A, Rivera, AA, Banerjee, NS, Moore, ST, Numnum, TM, Espinosa-de-los-Monteros, A, Richter, DF, Siegal, GP, Chow, LT, Feldman, D, Vasconez, LO, Mathis, JM, Stoff-Khalili, MA & Curiel, DT 2009, 'Promotion of incisional wound repair by human mesenchymal stem cell transplantation', Experimental Dermatology, vol. 18, no. 4, pp. 362-369. https://doi.org/10.1111/j.1600-0625.2008.00792.x

Promotion of incisional wound repair by human mesenchymal stem cell transplantation. / Stoff, Alexander; Rivera, Angel A.; Banerjee, N. Sanjib; Moore, Steven T.; Numnum, T. Michael; Espinosa-de-los-Monteros, Antonio; Richter, Dirk F.; Siegal, Gene P.; Chow, Louise T.; Feldman, Dale; Vasconez, Luis O.; Mathis, J. Michael; Stoff-Khalili, Mariam A.; Curiel, David T.

In: Experimental Dermatology, Vol. 18, No. 4, 01.04.2009, p. 362-369.

Research output: Contribution to journalArticle

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T1 - Promotion of incisional wound repair by human mesenchymal stem cell transplantation

AU - Stoff, Alexander

AU - Rivera, Angel A.

AU - Banerjee, N. Sanjib

AU - Moore, Steven T.

AU - Numnum, T. Michael

AU - Espinosa-de-los-Monteros, Antonio

AU - Richter, Dirk F.

AU - Siegal, Gene P.

AU - Chow, Louise T.

AU - Feldman, Dale

AU - Vasconez, Luis O.

AU - Mathis, J. Michael

AU - Stoff-Khalili, Mariam A.

AU - Curiel, David T.

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Stoff A, Rivera AA, Banerjee NS, Moore ST, Numnum TM, Espinosa-de-los-Monteros A et al. Promotion of incisional wound repair by human mesenchymal stem cell transplantation. Experimental Dermatology. 2009 Apr 1;18(4):362-369. https://doi.org/10.1111/j.1600-0625.2008.00792.x