TY - JOUR
T1 - Promoter methylation of E-cadherin, p16, and RAR-β2 genes in breast tumors and dietary intake of nutrients important in one-carbon metabolism
AU - Tao, Meng Hua
AU - Mason, Joel B.
AU - Marian, Catalin
AU - McCann, Susan E.
AU - Platek, Mary E.
AU - Millen, Amy
AU - Ambrosone, Christine
AU - Edge, Stephen B.
AU - Krishnan, Shiva S.
AU - Trevisan, Maurizio
AU - Shields, Peter G.
AU - Freudenheim, Jo L.
N1 - Funding Information:
This study would not have been possible without the support of all the study participants and the research staff of the Western New York Exposures and Breast Cancer (WEB) Study. This work was supported in part by the National Institute on Alcohol Abuse and Alcoholism (P50-AA09802), the Department of Defense (DAMD 179616202, DAMD 17030446), and United States Public Health Service (USPHS) from the National Cancer Institute (R01CA 092040).
PY - 2011/10
Y1 - 2011/10
N2 - Aberrant DNA methylation plays a critical role in carcinogenesis, and the availability of dietary factors involved in 1-carbon metabolism may contribute to aberrant DNA methylation. We investigated the association of intake of folate, vitamins B2, B6, B12, and methionine with promoter methylation of E-cadherin, p16, and RAR-β2 genes in archived tumor tissues from incident, primary breast cancer cases in a population-based case-control study. Real-time methylation-specific PCR was performed on 803 paraffin-embedded samples; usual dietary intake was queried from a food frequency questionnaire. Unconditional logistic regression was used to derive adjusted odds ratios and 95% confidence intervals for likelihood of promoter methylation for high compared to low intake of those 1-carbon nutrients. Overall, in case-case comparisons, dietary intakes of folate, vitamins B2, B6, B12, and methionine were not associated with likelihood of promoter methylation of E- cadherin, p16, and RAR-β2 for all cases combined or within strata defined by menopausal status and estrogen receptor status in this study. This finding, however, does not exclude the possibility that intake of such nutrients might have the ability to modulate promoter methylation in normal or premalignant (dysplastic) breast tissue.
AB - Aberrant DNA methylation plays a critical role in carcinogenesis, and the availability of dietary factors involved in 1-carbon metabolism may contribute to aberrant DNA methylation. We investigated the association of intake of folate, vitamins B2, B6, B12, and methionine with promoter methylation of E-cadherin, p16, and RAR-β2 genes in archived tumor tissues from incident, primary breast cancer cases in a population-based case-control study. Real-time methylation-specific PCR was performed on 803 paraffin-embedded samples; usual dietary intake was queried from a food frequency questionnaire. Unconditional logistic regression was used to derive adjusted odds ratios and 95% confidence intervals for likelihood of promoter methylation for high compared to low intake of those 1-carbon nutrients. Overall, in case-case comparisons, dietary intakes of folate, vitamins B2, B6, B12, and methionine were not associated with likelihood of promoter methylation of E- cadherin, p16, and RAR-β2 for all cases combined or within strata defined by menopausal status and estrogen receptor status in this study. This finding, however, does not exclude the possibility that intake of such nutrients might have the ability to modulate promoter methylation in normal or premalignant (dysplastic) breast tissue.
UR - http://www.scopus.com/inward/record.url?scp=80053637383&partnerID=8YFLogxK
U2 - 10.1080/01635581.2011.605982
DO - 10.1080/01635581.2011.605982
M3 - Article
C2 - 21916701
AN - SCOPUS:80053637383
SN - 0163-5581
VL - 63
SP - 1143
EP - 1150
JO - Nutrition and Cancer
JF - Nutrition and Cancer
IS - 7
ER -