Proliferative capacity of stem/progenitor-like cells in the kidney may associate with the outcome of patients with acute tubular necrosis

Youxin Ye, Bingyin Wang, Xinxin Jiang, Weiming Hu, Jian Feng, Hua Li, Mei Jin, Yingjuan Ying, Wenjuan Wang, Xiaoou Mao, Kunlin Jin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Animal studies indicate that adult renal stem/progenitor cells can undergo rapid proliferation in response to renal injury, but whether the same is true in humans is largely unknown. To examine the profile of renal stem/progenitor cells responsible for acute tubular necrosis in human kidney, double and triple immunostaining was performed using proliferative marker and stem/progenitor protein markers on sections from 10 kidneys with acute tubular necrosis and 4 normal adult kidneys. The immunopositive cells were recorded using 2-photon confocal laser scanning microscopy. We found that dividing cells were present in the tubules of the cortex and medulla, as well as the glomerulus in normal human kidney. Proliferative cells in the parietal layer of Bowman capsule expressed CD133, and dividing cells in the tubules expressed immature cell protein markers paired box gene 2, vimentin, and nestin. After acute tubular necrosis, Ki67-positive cells in the cortex tubules significantly increased compared with normal adult kidney. These Ki67-positive cells expressed CD133 and paired box gene 2, but not the cell death marker, activated caspase-3. In addition, the number of dividing cells increased significantly in patients with acute tubular necrosis who subsequently recovered, compared with patients with acute tubular necrosis who consequently developed protracted acute tubular necrosis or died. Our data suggest that renal stem/progenitor cells may reside not only in the parietal layer of Bowman capsule but also in the cortex and medulla in normal human kidney, and the proliferative capacity of renal stem/progenitor cells after acute tubular necrosis may be an important determinant of a patient's outcome.

Original languageEnglish
Pages (from-to)1132-1141
Number of pages10
JournalHuman Pathology
Volume42
Issue number8
DOIs
StatePublished - 1 Aug 2011

Fingerprint

Necrosis
Stem Cells
Kidney
Bowman Capsule
PAX2 Transcription Factor
Acute Kidney Tubular Necrosis
Nestin
Vimentin
Photons
Confocal Microscopy
Caspase 3
Cell Death
Cell Count
Wounds and Injuries
Genes
Proteins

Keywords

  • ATN
  • Kidney
  • Outcome
  • Progenitor cells
  • Proliferation
  • Stem cells

Cite this

Ye, Youxin ; Wang, Bingyin ; Jiang, Xinxin ; Hu, Weiming ; Feng, Jian ; Li, Hua ; Jin, Mei ; Ying, Yingjuan ; Wang, Wenjuan ; Mao, Xiaoou ; Jin, Kunlin. / Proliferative capacity of stem/progenitor-like cells in the kidney may associate with the outcome of patients with acute tubular necrosis. In: Human Pathology. 2011 ; Vol. 42, No. 8. pp. 1132-1141.
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Proliferative capacity of stem/progenitor-like cells in the kidney may associate with the outcome of patients with acute tubular necrosis. / Ye, Youxin; Wang, Bingyin; Jiang, Xinxin; Hu, Weiming; Feng, Jian; Li, Hua; Jin, Mei; Ying, Yingjuan; Wang, Wenjuan; Mao, Xiaoou; Jin, Kunlin.

In: Human Pathology, Vol. 42, No. 8, 01.08.2011, p. 1132-1141.

Research output: Contribution to journalArticle

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T1 - Proliferative capacity of stem/progenitor-like cells in the kidney may associate with the outcome of patients with acute tubular necrosis

AU - Ye, Youxin

AU - Wang, Bingyin

AU - Jiang, Xinxin

AU - Hu, Weiming

AU - Feng, Jian

AU - Li, Hua

AU - Jin, Mei

AU - Ying, Yingjuan

AU - Wang, Wenjuan

AU - Mao, Xiaoou

AU - Jin, Kunlin

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N2 - Animal studies indicate that adult renal stem/progenitor cells can undergo rapid proliferation in response to renal injury, but whether the same is true in humans is largely unknown. To examine the profile of renal stem/progenitor cells responsible for acute tubular necrosis in human kidney, double and triple immunostaining was performed using proliferative marker and stem/progenitor protein markers on sections from 10 kidneys with acute tubular necrosis and 4 normal adult kidneys. The immunopositive cells were recorded using 2-photon confocal laser scanning microscopy. We found that dividing cells were present in the tubules of the cortex and medulla, as well as the glomerulus in normal human kidney. Proliferative cells in the parietal layer of Bowman capsule expressed CD133, and dividing cells in the tubules expressed immature cell protein markers paired box gene 2, vimentin, and nestin. After acute tubular necrosis, Ki67-positive cells in the cortex tubules significantly increased compared with normal adult kidney. These Ki67-positive cells expressed CD133 and paired box gene 2, but not the cell death marker, activated caspase-3. In addition, the number of dividing cells increased significantly in patients with acute tubular necrosis who subsequently recovered, compared with patients with acute tubular necrosis who consequently developed protracted acute tubular necrosis or died. Our data suggest that renal stem/progenitor cells may reside not only in the parietal layer of Bowman capsule but also in the cortex and medulla in normal human kidney, and the proliferative capacity of renal stem/progenitor cells after acute tubular necrosis may be an important determinant of a patient's outcome.

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