Production of interleukin-18 in human tuberculosis

Ramakrishna Vankayalapati; Benjamin Wizel; Stephen E. Weis; Buka Samten; William M. Girard; Peter F. Barnes

doi:10.1086/315656

Production of interleukin-18 in human tuberculosis

Ramakrishna Vankayalapati, Benjamin Wizel, Stephen E. Weis, Buka Samten, William M. Girard, Peter F. Barnes

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Abstract

To investigate the role of interleukin (IL)-18 in human tuberculosis, IL-18 production was evaluated in blood and at the site of disease in patients with tuberculosis. Mycobacterium tuberculosis-stimulated peripheral blood mononuclear cells (PBMC) from tuberculosis patients secreted less IL-18 and interferon-γ (IFN-γ) than did PBMC from healthy persons reactive to tuberculin. M. tuberculosis-induced IFN-γ production was inhibited by anti- IL-18 and enhanced by recombinant IL-18. Alveolar macrophages secreted IL-18 in response to M. tuberculosis, and IL-18 and IFN-γ concentrations were higher in pleural fluid of patients with tuberculosis than in pleural fluid of patients with nontuberculous diseases. These findings demonstrate that IL- 18 production by PBMC correlates with IFN-γ production and effective immunity to tuberculosis, suggesting that IL-18 contributes to a protective type 1 cytokine response in persons with mycobacterial infection.

Original language	English
Pages (from-to)	234-239
Number of pages	6
Journal	Journal of Infectious Diseases
Volume	182
Issue number	1
DOIs	https://doi.org/10.1086/315656
State	Published - 2000

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abstract = "To investigate the role of interleukin (IL)-18 in human tuberculosis, IL-18 production was evaluated in blood and at the site of disease in patients with tuberculosis. Mycobacterium tuberculosis-stimulated peripheral blood mononuclear cells (PBMC) from tuberculosis patients secreted less IL-18 and interferon-γ (IFN-γ) than did PBMC from healthy persons reactive to tuberculin. M. tuberculosis-induced IFN-γ production was inhibited by anti- IL-18 and enhanced by recombinant IL-18. Alveolar macrophages secreted IL-18 in response to M. tuberculosis, and IL-18 and IFN-γ concentrations were higher in pleural fluid of patients with tuberculosis than in pleural fluid of patients with nontuberculous diseases. These findings demonstrate that IL- 18 production by PBMC correlates with IFN-γ production and effective immunity to tuberculosis, suggesting that IL-18 contributes to a protective type 1 cytokine response in persons with mycobacterial infection.",

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AU - Vankayalapati, Ramakrishna

AU - Wizel, Benjamin

AU - Weis, Stephen E.

AU - Samten, Buka

AU - Girard, William M.

AU - Barnes, Peter F.

PY - 2000

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AB - To investigate the role of interleukin (IL)-18 in human tuberculosis, IL-18 production was evaluated in blood and at the site of disease in patients with tuberculosis. Mycobacterium tuberculosis-stimulated peripheral blood mononuclear cells (PBMC) from tuberculosis patients secreted less IL-18 and interferon-γ (IFN-γ) than did PBMC from healthy persons reactive to tuberculin. M. tuberculosis-induced IFN-γ production was inhibited by anti- IL-18 and enhanced by recombinant IL-18. Alveolar macrophages secreted IL-18 in response to M. tuberculosis, and IL-18 and IFN-γ concentrations were higher in pleural fluid of patients with tuberculosis than in pleural fluid of patients with nontuberculous diseases. These findings demonstrate that IL- 18 production by PBMC correlates with IFN-γ production and effective immunity to tuberculosis, suggesting that IL-18 contributes to a protective type 1 cytokine response in persons with mycobacterial infection.

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Production of interleukin-18 in human tuberculosis

Abstract

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