Preliminary Assessment of Microbiome Changes Following Blood-Feeding and Survivorship in the Amblyomma americanum Nymph-to-Adult Transition using Semiconductor Sequencing

Arturo C. Menchaca, David K. Visi, Otto F. Strey, Pete D. Teel, Kevin Kalinowski, Michael S. Allen, Phillip C. Williamson

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The physiology of ticks supports a diverse community of non-pathogenic and pathogenic organisms. This study aims to initially characterize the microbial community present within colony-reared Amblyomma americanum using PCR of the variable region 5 of the 16S rRNA gene followed by semiconductor sequencing and classification of sequence data using the Ribosomal Database Project and MG-RAST analysis tools. Comparison of amplicon library datasets revealed changes in the microbiomes in newly engorged nymphs, newly-molted adults, and aged adults, as well as ticks exposed to different environmental conditions. These preliminary data support the concept that microbe survivorship and diversity are partially dependent upon environmental variables and the sequence of blood feeding, molting, and aging. The maintenance and/or emergence of pathogens in ticks may be dependent in part on temporal changes in the microbial community of the tick microbiome.

Original languageEnglish
Article numbere67129
JournalPLoS ONE
Volume8
Issue number6
DOIs
StatePublished - 24 Jun 2013

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semiconductors
Amblyomma americanum
Nymph
Semiconductors
Microbiota
Physiology
Ticks
Pathogens
nymphs
ticks
Blood
Survival Rate
Genes
Aging of materials
survival rate
Semiconductor materials
blood
microbial communities
Molting
environmental factors

Cite this

Menchaca, Arturo C. ; Visi, David K. ; Strey, Otto F. ; Teel, Pete D. ; Kalinowski, Kevin ; Allen, Michael S. ; Williamson, Phillip C. / Preliminary Assessment of Microbiome Changes Following Blood-Feeding and Survivorship in the Amblyomma americanum Nymph-to-Adult Transition using Semiconductor Sequencing. In: PLoS ONE. 2013 ; Vol. 8, No. 6.
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Preliminary Assessment of Microbiome Changes Following Blood-Feeding and Survivorship in the Amblyomma americanum Nymph-to-Adult Transition using Semiconductor Sequencing. / Menchaca, Arturo C.; Visi, David K.; Strey, Otto F.; Teel, Pete D.; Kalinowski, Kevin; Allen, Michael S.; Williamson, Phillip C.

In: PLoS ONE, Vol. 8, No. 6, e67129, 24.06.2013.

Research output: Contribution to journalArticle

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