PRDM1 is directly targeted by miR-30a-5p and modulates the Wnt/β-catenin pathway in a Dkk1-dependent manner during glioma growth

Xuan Wang, Kun Wang, Lei Han, Anling Zhang, Zhendong Shi, Kailiang Zhang, Hongwei Zhang, Shaohua Yang, Peiyu Pu, Changhong Shen, Chunjiang Yu, Chunsheng Kang

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Abstract

The transcriptional regulator PRDM1 controls cell-fate decisions and has been implicated in human tumorigenesis as a tumor suppressor. However, its pathological role in glioma remains elusive. In this study, we showed that PRDM1 protein levels were inversely correlated with the pathological grade of gliomas and were predictive of patient survival in a retrospective analysis. Restored expression of PRDM1 inhibited proliferation and suppressed invasion by glioma cells. Mechanistic investigation revealed that PRDM1 attenuated glioma malignancy by negatively modulating Wnt/β-catenin signaling and this modulation was dependent on the Wnt inhibitor Dkk1. Using bioinformatics and biological approaches, we found that PRDM1 was a direct target of miR-30a-5p, and PRDM1 dysfunction was attributable to miR-30a-5p-mediated repression. Our results provide evidence that PRDM1 deficiency contributes to the phenotype maintenance and pathogenesis of gliomas.

Original languageEnglish
Pages (from-to)211-219
Number of pages9
JournalCancer Letters
Volume331
Issue number2
DOIs
StatePublished - 1 May 2013

Fingerprint

Catenins
Wnt Signaling Pathway
Glioma
Growth
Computational Biology
Neoplasms
Carcinogenesis
Maintenance
Phenotype
Survival
Proteins

Keywords

  • Dkk1
  • Glioma
  • MiR-30a-5p
  • PRDM1
  • β-Catenin

Cite this

Wang, Xuan ; Wang, Kun ; Han, Lei ; Zhang, Anling ; Shi, Zhendong ; Zhang, Kailiang ; Zhang, Hongwei ; Yang, Shaohua ; Pu, Peiyu ; Shen, Changhong ; Yu, Chunjiang ; Kang, Chunsheng. / PRDM1 is directly targeted by miR-30a-5p and modulates the Wnt/β-catenin pathway in a Dkk1-dependent manner during glioma growth. In: Cancer Letters. 2013 ; Vol. 331, No. 2. pp. 211-219.
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title = "PRDM1 is directly targeted by miR-30a-5p and modulates the Wnt/β-catenin pathway in a Dkk1-dependent manner during glioma growth",
abstract = "The transcriptional regulator PRDM1 controls cell-fate decisions and has been implicated in human tumorigenesis as a tumor suppressor. However, its pathological role in glioma remains elusive. In this study, we showed that PRDM1 protein levels were inversely correlated with the pathological grade of gliomas and were predictive of patient survival in a retrospective analysis. Restored expression of PRDM1 inhibited proliferation and suppressed invasion by glioma cells. Mechanistic investigation revealed that PRDM1 attenuated glioma malignancy by negatively modulating Wnt/β-catenin signaling and this modulation was dependent on the Wnt inhibitor Dkk1. Using bioinformatics and biological approaches, we found that PRDM1 was a direct target of miR-30a-5p, and PRDM1 dysfunction was attributable to miR-30a-5p-mediated repression. Our results provide evidence that PRDM1 deficiency contributes to the phenotype maintenance and pathogenesis of gliomas.",
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author = "Xuan Wang and Kun Wang and Lei Han and Anling Zhang and Zhendong Shi and Kailiang Zhang and Hongwei Zhang and Shaohua Yang and Peiyu Pu and Changhong Shen and Chunjiang Yu and Chunsheng Kang",
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Wang, X, Wang, K, Han, L, Zhang, A, Shi, Z, Zhang, K, Zhang, H, Yang, S, Pu, P, Shen, C, Yu, C & Kang, C 2013, 'PRDM1 is directly targeted by miR-30a-5p and modulates the Wnt/β-catenin pathway in a Dkk1-dependent manner during glioma growth', Cancer Letters, vol. 331, no. 2, pp. 211-219. https://doi.org/10.1016/j.canlet.2013.01.005

PRDM1 is directly targeted by miR-30a-5p and modulates the Wnt/β-catenin pathway in a Dkk1-dependent manner during glioma growth. / Wang, Xuan; Wang, Kun; Han, Lei; Zhang, Anling; Shi, Zhendong; Zhang, Kailiang; Zhang, Hongwei; Yang, Shaohua; Pu, Peiyu; Shen, Changhong; Yu, Chunjiang; Kang, Chunsheng.

In: Cancer Letters, Vol. 331, No. 2, 01.05.2013, p. 211-219.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Zhang, Anling

AU - Shi, Zhendong

AU - Zhang, Kailiang

AU - Zhang, Hongwei

AU - Yang, Shaohua

AU - Pu, Peiyu

AU - Shen, Changhong

AU - Yu, Chunjiang

AU - Kang, Chunsheng

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AB - The transcriptional regulator PRDM1 controls cell-fate decisions and has been implicated in human tumorigenesis as a tumor suppressor. However, its pathological role in glioma remains elusive. In this study, we showed that PRDM1 protein levels were inversely correlated with the pathological grade of gliomas and were predictive of patient survival in a retrospective analysis. Restored expression of PRDM1 inhibited proliferation and suppressed invasion by glioma cells. Mechanistic investigation revealed that PRDM1 attenuated glioma malignancy by negatively modulating Wnt/β-catenin signaling and this modulation was dependent on the Wnt inhibitor Dkk1. Using bioinformatics and biological approaches, we found that PRDM1 was a direct target of miR-30a-5p, and PRDM1 dysfunction was attributable to miR-30a-5p-mediated repression. Our results provide evidence that PRDM1 deficiency contributes to the phenotype maintenance and pathogenesis of gliomas.

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