Potentiation of glutamate-activated currents in isolated hippocampal neurons

Yuri I. Zilberter; Vladimir V. Uteshev; Svetlana N. Sokolova; Leonid G. Motin; Haikaz H. Eremjan

doi:10.1016/0896-6273(90)90214-Z

Potentiation of glutamate-activated currents in isolated hippocampal neurons

Yuri I. Zilberter, Vladimir V. Uteshev, Svetlana N. Sokolova, Leonid G. Motin, Haikaz H. Eremjan

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Abstract

"Fast chemical stimulation" was shown to induce potentiation of glutamate-activated currents in neurons isolated from rat hippocampus. A fast application system allowed solution changes up to a rate of 20 Hz. In Mg²⁺-free solution, the response to glutamate application immediately after repetitive stimulation with glutamate plus glycine was increased by 25%-88%, returning to control levels over 10-15 min. Enhancement of glutamate-induced currents was also seen after stimulation with solutions containing aspartate or NMDA plus glycine. Aspartate-induced currents were not potentiated. These and other observations demonstrate that in a purely "postsynaptic" system, short-term potentiation can be induced and is mediated via NMDA receptors whereas the potentiated current is carried via non-NMDA glutamate receptor channels.

Original language	English
Pages (from-to)	597-602
Number of pages	6
Journal	Neuron
Volume	5
Issue number	5
DOIs	https://doi.org/10.1016/0896-6273(90)90214-Z
State	Published - Nov 1990

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title = "Potentiation of glutamate-activated currents in isolated hippocampal neurons",

abstract = "{"}Fast chemical stimulation{"} was shown to induce potentiation of glutamate-activated currents in neurons isolated from rat hippocampus. A fast application system allowed solution changes up to a rate of 20 Hz. In Mg2+-free solution, the response to glutamate application immediately after repetitive stimulation with glutamate plus glycine was increased by 25%-88%, returning to control levels over 10-15 min. Enhancement of glutamate-induced currents was also seen after stimulation with solutions containing aspartate or NMDA plus glycine. Aspartate-induced currents were not potentiated. These and other observations demonstrate that in a purely {"}postsynaptic{"} system, short-term potentiation can be induced and is mediated via NMDA receptors whereas the potentiated current is carried via non-NMDA glutamate receptor channels.",

author = "Zilberter, {Yuri I.} and Uteshev, {Vladimir V.} and Sokolova, {Svetlana N.} and Motin, {Leonid G.} and Eremjan, {Haikaz H.}",

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T1 - Potentiation of glutamate-activated currents in isolated hippocampal neurons

AU - Zilberter, Yuri I.

AU - Uteshev, Vladimir V.

AU - Sokolova, Svetlana N.

AU - Motin, Leonid G.

AU - Eremjan, Haikaz H.

PY - 1990/11

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N2 - "Fast chemical stimulation" was shown to induce potentiation of glutamate-activated currents in neurons isolated from rat hippocampus. A fast application system allowed solution changes up to a rate of 20 Hz. In Mg2+-free solution, the response to glutamate application immediately after repetitive stimulation with glutamate plus glycine was increased by 25%-88%, returning to control levels over 10-15 min. Enhancement of glutamate-induced currents was also seen after stimulation with solutions containing aspartate or NMDA plus glycine. Aspartate-induced currents were not potentiated. These and other observations demonstrate that in a purely "postsynaptic" system, short-term potentiation can be induced and is mediated via NMDA receptors whereas the potentiated current is carried via non-NMDA glutamate receptor channels.

AB - "Fast chemical stimulation" was shown to induce potentiation of glutamate-activated currents in neurons isolated from rat hippocampus. A fast application system allowed solution changes up to a rate of 20 Hz. In Mg2+-free solution, the response to glutamate application immediately after repetitive stimulation with glutamate plus glycine was increased by 25%-88%, returning to control levels over 10-15 min. Enhancement of glutamate-induced currents was also seen after stimulation with solutions containing aspartate or NMDA plus glycine. Aspartate-induced currents were not potentiated. These and other observations demonstrate that in a purely "postsynaptic" system, short-term potentiation can be induced and is mediated via NMDA receptors whereas the potentiated current is carried via non-NMDA glutamate receptor channels.

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Potentiation of glutamate-activated currents in isolated hippocampal neurons

Abstract

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