Positive selection of an H2-M3 restricted T cell receptor

Rance E. Berg, Michael F. Princiotta, Stefan Irion, Juli A. Moticka, Kevin R. Dahl, Uwe D. Staerz

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Abstract

Thymocytes are positively selected for β T cell antigen receptors (TCR) that recognize antigen in conjunction with self-major histocompatibility complex (MHC) molecules. MHC bound peptides participate in positive selection; however, their role has remained controversial. A TCR transgenic mouse was established using a TCR restricted to the MHC class Ib molecule, H2-M3. Having defined H2-M3 as the positively selecting MHC molecule, the severely limited number of H2-M3 binding peptides allowed us to characterize an NADH dehydrogenase subunit 1 (ND1)-derived peptide as the physiological ligand of positive selection. This peptide bears no apparent sequence homology to the cognate peptide, is expressed ubiquitously, and yet does not interfere with peripheral T cells. Our studies also suggest that positive selection becomes promiscuous at high epitope densities.

Original languageEnglish
Pages (from-to)33-43
Number of pages11
JournalImmunity
Volume11
Issue number1
DOIs
StatePublished - Jul 1999

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    Berg, R. E., Princiotta, M. F., Irion, S., Moticka, J. A., Dahl, K. R., & Staerz, U. D. (1999). Positive selection of an H2-M3 restricted T cell receptor. Immunity, 11(1), 33-43. https://doi.org/10.1016/S1074-7613(00)80079-5