@article{9a45babb86ca4d9d9130a0bcb38bbc49,
title = "Positive selection of an H2-M3 restricted T cell receptor",
abstract = "Thymocytes are positively selected for β T cell antigen receptors (TCR) that recognize antigen in conjunction with self-major histocompatibility complex (MHC) molecules. MHC bound peptides participate in positive selection; however, their role has remained controversial. A TCR transgenic mouse was established using a TCR restricted to the MHC class Ib molecule, H2-M3. Having defined H2-M3 as the positively selecting MHC molecule, the severely limited number of H2-M3 binding peptides allowed us to characterize an NADH dehydrogenase subunit 1 (ND1)-derived peptide as the physiological ligand of positive selection. This peptide bears no apparent sequence homology to the cognate peptide, is expressed ubiquitously, and yet does not interfere with peripheral T cells. Our studies also suggest that positive selection becomes promiscuous at high epitope densities.",
author = "Berg, {Rance E.} and Princiotta, {Michael F.} and Stefan Irion and Moticka, {Juli A.} and Dahl, {Kevin R.} and Staerz, {Uwe D.}",
note = "Funding Information: We would like to thank Dean Becker and Gail Ackermann for help in making the C10.4 TCR trans + mouse; Steve Rozzo for assistance with microsatellite mapping; Dehua Pei for generous donation of the peptide deformylase; Kirsten Fischer Lindahl for providing the B6.R9 mice and the anti-ND1α clones; Fred Alt for the RAG2 −/− mice; John H. Freed for assistance with generating the peptide extract; Amy Marrs and Randal Anselment for primer and peptide synthesis; Robert Rich and John Rodgers for the 13S2 cell line, John W. Kappler and Terry A. Potter for helpful discussions; and Dimitris Kiousis for the CD2 based minigene construct. This work was supported in part by grants HD26841, AI35194 and AI22295 from the National Institutes of Health (U. D. S.).",
year = "1999",
month = jul,
doi = "10.1016/S1074-7613(00)80079-5",
language = "English",
volume = "11",
pages = "33--43",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "1",
}