Polarized fluorescent nanospheres

Rafal Luchowski, Zygmunt Gryczynski, Zeno Földes-Papp, Aaron Chang, Julian Borejdo, Pabak Sarkar, Ignacy Gryczynski

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study. We demonstrate that photoselective bleaching creates beads with highly polarized fluorescence. First, the beads were immobilized in a PVA polymer. Second, the beads-doped PVA film was exposed to the illumination within the dye absorption band. A progressive decrease of absorption was observed. Next, photophysical properties of photobleached and not bleached films dissolved in water were compared.

Original languageEnglish
Pages (from-to)4289-4299
Number of pages11
JournalOptics Express
Volume18
Issue number5
DOIs
StatePublished - 1 Mar 2010

Fingerprint

beads
dyes
fluorescence
nanoparticles
polarization
bleaching
statistical distributions
illumination
microscopy
absorption spectra
polymers
water
spectroscopy

Cite this

Luchowski, Rafal ; Gryczynski, Zygmunt ; Földes-Papp, Zeno ; Chang, Aaron ; Borejdo, Julian ; Sarkar, Pabak ; Gryczynski, Ignacy. / Polarized fluorescent nanospheres. In: Optics Express. 2010 ; Vol. 18, No. 5. pp. 4289-4299.
@article{bf0a6bf7cfc94e1591d5f8c623291924,
title = "Polarized fluorescent nanospheres",
abstract = "Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study. We demonstrate that photoselective bleaching creates beads with highly polarized fluorescence. First, the beads were immobilized in a PVA polymer. Second, the beads-doped PVA film was exposed to the illumination within the dye absorption band. A progressive decrease of absorption was observed. Next, photophysical properties of photobleached and not bleached films dissolved in water were compared.",
author = "Rafal Luchowski and Zygmunt Gryczynski and Zeno F{\"o}ldes-Papp and Aaron Chang and Julian Borejdo and Pabak Sarkar and Ignacy Gryczynski",
year = "2010",
month = "3",
day = "1",
doi = "10.1364/OE.18.004289",
language = "English",
volume = "18",
pages = "4289--4299",
journal = "Optics Express",
issn = "1094-4087",
publisher = "The Optical Society",
number = "5",

}

Luchowski, R, Gryczynski, Z, Földes-Papp, Z, Chang, A, Borejdo, J, Sarkar, P & Gryczynski, I 2010, 'Polarized fluorescent nanospheres', Optics Express, vol. 18, no. 5, pp. 4289-4299. https://doi.org/10.1364/OE.18.004289

Polarized fluorescent nanospheres. / Luchowski, Rafal; Gryczynski, Zygmunt; Földes-Papp, Zeno; Chang, Aaron; Borejdo, Julian; Sarkar, Pabak; Gryczynski, Ignacy.

In: Optics Express, Vol. 18, No. 5, 01.03.2010, p. 4289-4299.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Polarized fluorescent nanospheres

AU - Luchowski, Rafal

AU - Gryczynski, Zygmunt

AU - Földes-Papp, Zeno

AU - Chang, Aaron

AU - Borejdo, Julian

AU - Sarkar, Pabak

AU - Gryczynski, Ignacy

PY - 2010/3/1

Y1 - 2010/3/1

N2 - Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study. We demonstrate that photoselective bleaching creates beads with highly polarized fluorescence. First, the beads were immobilized in a PVA polymer. Second, the beads-doped PVA film was exposed to the illumination within the dye absorption band. A progressive decrease of absorption was observed. Next, photophysical properties of photobleached and not bleached films dissolved in water were compared.

AB - Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study. We demonstrate that photoselective bleaching creates beads with highly polarized fluorescence. First, the beads were immobilized in a PVA polymer. Second, the beads-doped PVA film was exposed to the illumination within the dye absorption band. A progressive decrease of absorption was observed. Next, photophysical properties of photobleached and not bleached films dissolved in water were compared.

UR - http://www.scopus.com/inward/record.url?scp=77749258427&partnerID=8YFLogxK

U2 - 10.1364/OE.18.004289

DO - 10.1364/OE.18.004289

M3 - Article

C2 - 20389440

AN - SCOPUS:77749258427

VL - 18

SP - 4289

EP - 4299

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 5

ER -

Luchowski R, Gryczynski Z, Földes-Papp Z, Chang A, Borejdo J, Sarkar P et al. Polarized fluorescent nanospheres. Optics Express. 2010 Mar 1;18(5):4289-4299. https://doi.org/10.1364/OE.18.004289