Polarized fluorescent nanospheres

Rafal Luchowski, Zygmunt Gryczynski, Zeno Földes-Papp, Aaron Chang, Julian Borejdo, Pabak Sarkar, Ignacy Gryczynski

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study. We demonstrate that photoselective bleaching creates beads with highly polarized fluorescence. First, the beads were immobilized in a PVA polymer. Second, the beads-doped PVA film was exposed to the illumination within the dye absorption band. A progressive decrease of absorption was observed. Next, photophysical properties of photobleached and not bleached films dissolved in water were compared.

Original languageEnglish
Pages (from-to)4289-4299
Number of pages11
JournalOptics Express
Volume18
Issue number5
DOIs
StatePublished - 1 Mar 2010

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