Polarization sensing of fluorophores in tissues for drug compliance monitoring

Zygmunt Gryczynski, Omoefe O. Abugo, Joseph R. Lakowicz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We used a new method, polarization sensing, to monitor the concentration of the fluorophore rhodamine 800 in an intralipid suspension and in chicken tissue. Rhodamine 800 (Rh800) could be excited at 648 nm using a laser pointer. We developed a simple device for measuring the combined emission from a highly polarized reference film and the unpolarized or orthogonally polarized emission of Rh800 from the scattering intralipid or tissue. The concentration of Rh800 in this medium was revealed by large changes in the polarization (P) with values of P ranging from 0.8 to -0.9. It is possible to vary the sensitive Rh800 concentration range by variation of the detected emission wavelengths, orientation of the excitation polarizer, or fluorophore concentration in the reference film. Polarization sensing of fluorophores in tissue requires only steady-state detection, and can be accomplished with simple and/or portable electronics. Such devices may find use in electronic detection of ingested medicines based on transdermal detection of nontoxic long-wavelength fluorophores.

Original languageEnglish
Pages (from-to)204-211
Number of pages8
JournalAnalytical Biochemistry
Volume273
Issue number2
DOIs
StatePublished - 10 Sep 1999

Fingerprint

Fluorophores
Drug Monitoring
Compliance
Polarization
Tissue
Monitoring
Pharmaceutical Preparations
Equipment and Supplies
Wavelength
Medicine
Chickens
Suspensions
Lasers
Electronic equipment
rhodamine 800
Scattering
phospholipid emulsion soybean oil

Cite this

Gryczynski, Zygmunt ; Abugo, Omoefe O. ; Lakowicz, Joseph R. / Polarization sensing of fluorophores in tissues for drug compliance monitoring. In: Analytical Biochemistry. 1999 ; Vol. 273, No. 2. pp. 204-211.
@article{f2fb93a04d3045b58ec436ed441a1022,
title = "Polarization sensing of fluorophores in tissues for drug compliance monitoring",
abstract = "We used a new method, polarization sensing, to monitor the concentration of the fluorophore rhodamine 800 in an intralipid suspension and in chicken tissue. Rhodamine 800 (Rh800) could be excited at 648 nm using a laser pointer. We developed a simple device for measuring the combined emission from a highly polarized reference film and the unpolarized or orthogonally polarized emission of Rh800 from the scattering intralipid or tissue. The concentration of Rh800 in this medium was revealed by large changes in the polarization (P) with values of P ranging from 0.8 to -0.9. It is possible to vary the sensitive Rh800 concentration range by variation of the detected emission wavelengths, orientation of the excitation polarizer, or fluorophore concentration in the reference film. Polarization sensing of fluorophores in tissue requires only steady-state detection, and can be accomplished with simple and/or portable electronics. Such devices may find use in electronic detection of ingested medicines based on transdermal detection of nontoxic long-wavelength fluorophores.",
author = "Zygmunt Gryczynski and Abugo, {Omoefe O.} and Lakowicz, {Joseph R.}",
year = "1999",
month = "9",
day = "10",
doi = "10.1006/abio.1999.4213",
language = "English",
volume = "273",
pages = "204--211",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",

}

Polarization sensing of fluorophores in tissues for drug compliance monitoring. / Gryczynski, Zygmunt; Abugo, Omoefe O.; Lakowicz, Joseph R.

In: Analytical Biochemistry, Vol. 273, No. 2, 10.09.1999, p. 204-211.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Polarization sensing of fluorophores in tissues for drug compliance monitoring

AU - Gryczynski, Zygmunt

AU - Abugo, Omoefe O.

AU - Lakowicz, Joseph R.

PY - 1999/9/10

Y1 - 1999/9/10

N2 - We used a new method, polarization sensing, to monitor the concentration of the fluorophore rhodamine 800 in an intralipid suspension and in chicken tissue. Rhodamine 800 (Rh800) could be excited at 648 nm using a laser pointer. We developed a simple device for measuring the combined emission from a highly polarized reference film and the unpolarized or orthogonally polarized emission of Rh800 from the scattering intralipid or tissue. The concentration of Rh800 in this medium was revealed by large changes in the polarization (P) with values of P ranging from 0.8 to -0.9. It is possible to vary the sensitive Rh800 concentration range by variation of the detected emission wavelengths, orientation of the excitation polarizer, or fluorophore concentration in the reference film. Polarization sensing of fluorophores in tissue requires only steady-state detection, and can be accomplished with simple and/or portable electronics. Such devices may find use in electronic detection of ingested medicines based on transdermal detection of nontoxic long-wavelength fluorophores.

AB - We used a new method, polarization sensing, to monitor the concentration of the fluorophore rhodamine 800 in an intralipid suspension and in chicken tissue. Rhodamine 800 (Rh800) could be excited at 648 nm using a laser pointer. We developed a simple device for measuring the combined emission from a highly polarized reference film and the unpolarized or orthogonally polarized emission of Rh800 from the scattering intralipid or tissue. The concentration of Rh800 in this medium was revealed by large changes in the polarization (P) with values of P ranging from 0.8 to -0.9. It is possible to vary the sensitive Rh800 concentration range by variation of the detected emission wavelengths, orientation of the excitation polarizer, or fluorophore concentration in the reference film. Polarization sensing of fluorophores in tissue requires only steady-state detection, and can be accomplished with simple and/or portable electronics. Such devices may find use in electronic detection of ingested medicines based on transdermal detection of nontoxic long-wavelength fluorophores.

UR - http://www.scopus.com/inward/record.url?scp=0033543509&partnerID=8YFLogxK

U2 - 10.1006/abio.1999.4213

DO - 10.1006/abio.1999.4213

M3 - Article

C2 - 10469491

AN - SCOPUS:0033543509

VL - 273

SP - 204

EP - 211

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -