Polarization-based sensing of glucose using an oriented reference film

Joseph R. Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski, Leah Tolosa, Lisa Randers-Eichhorn, Govind Rao

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We describe a new approach to glucose sensing using polarization measurements in the presence of a stretch-oriented reference film. The method relies on measurement of the polarized emission from the reference film and of a fluorophore which changes intensity in response to glucose. A glucose-sensitive fluorescent signal was provided by the glucose/galactose binding protein from E. coli. This protein was labeled with an environmentally sensitive fluorophore at a single genetically inserted cysteine residue, and displayed decreased fluorescence upon glucose binding. Using the protein and the reference film we observed glucose-sensitive polarization values for micromolar glucose concentrations. This method of polarization-based sensing is generic and can be used for any sensing fluorophore which displays a change in intensity. In principle, one can construct simple and economical devices for this type of glucose measurement.

Original languageEnglish
Pages (from-to)443-449
Number of pages7
JournalJournal of Biomedical Optics
Volume4
Issue number4
DOIs
StatePublished - 1 Oct 1999

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glucose
Glucose
Polarization
polarization
Fluorophores
proteins
Proteins
galactose
cysteine
Escherichia coli
Cysteine
Fluorescence
fluorescence

Cite this

Lakowicz, Joseph R. ; Gryczynski, Ignacy ; Gryczynski, Zygmunt ; Tolosa, Leah ; Randers-Eichhorn, Lisa ; Rao, Govind. / Polarization-based sensing of glucose using an oriented reference film. In: Journal of Biomedical Optics. 1999 ; Vol. 4, No. 4. pp. 443-449.
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Polarization-based sensing of glucose using an oriented reference film. / Lakowicz, Joseph R.; Gryczynski, Ignacy; Gryczynski, Zygmunt; Tolosa, Leah; Randers-Eichhorn, Lisa; Rao, Govind.

In: Journal of Biomedical Optics, Vol. 4, No. 4, 01.10.1999, p. 443-449.

Research output: Contribution to journalArticle

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