Polarization-based sensing of glucose using an oriented reference film

Joseph R. Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski, Leah Tolosa, Lisa Randers-Eichhorn, Govind Rao

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We describe a new approach to glucose sensing using polarization measurements in the presence of a stretch-oriented reference film. The method relies on measurement of the polarized emission from the reference film and of a fluorophore which changes intensity in response to glucose. A glucose-sensitive fluorescent signal was provided by the glucose/galactose binding protein from E. coli. This protein was labeled with an environmentally sensitive fluorophore at a single genetically inserted cysteine residue, and displayed decreased fluorescence upon glucose binding. Using the protein and the reference film we observed glucose-sensitive polarization values for micromolar glucose concentrations. This method of polarization-based sensing is generic and can be used for any sensing fluorophore which displays a change in intensity. In principle, one can construct simple and economical devices for this type of glucose measurement.

Original languageEnglish
Pages (from-to)443-449
Number of pages7
JournalJournal of Biomedical Optics
Volume4
Issue number4
DOIs
StatePublished - Oct 1999

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