Polarization-based oxygen sensor

Ignacy Gryczynski, Zygmunt Gryczynski, Govind Rao, Joseph R. Lakowicz

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


A new approach to oxygen sensing based on the luminescence polarization observed from a novel type of sensor is described. The oxygen sensor consists of an oxygen-sensitive silicone film containing tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) chloride [Ru(dpp)3Cl2] and an oxygen-insensitive film of Styryl 7 in poly(vinyl alcohol). Polarizers are used to select orthogonally polarized emission components from Ru(dpp)3Cl2 and Styryl 7. The polarization of the combined emission was found to be highly sensitive to the partial pressure of oxygen. This method of polarization sensing is generic and can be used with any fluorophore which displays an analyte-dependent change in intensity.

Original languageEnglish
Pages (from-to)1041-1044
Number of pages4
Issue number7
StatePublished - 1999


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