Planktonic microbial profiling in water samples from a Brazilian Amazonian reservoir

Bianca C.A. Cabral, Luísa Hoffmann, Bruce Budowle, Turán P. Ürményi, Rodrigo S. Moura-Neto, Sandra M.F.O. Azevedo, Rosane Silva

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Our comprehension of the dynamics and diversity of freshwater planktonic bacterial communities is far from complete concerning the Brazilian Amazonian region. Therefore, reference studies are urgently needed. We mapped bacterial communities present in the planktonic communities of a freshwater artificial reservoir located in the western Amazonian basin. Two samples were obtained from rainy and dry seasons, the periods during which water quality and plankton diversity undergo the most significant changes. Hypervariable 16S rRNA and shotgun sequencing were performed to describe the first reference of a microbial community in an Amazonian lentic system. Microbial composition consisted mainly of Betaproteobacteria, Cyanobacteria, Alphaproteobacteria, and Actinobacteria in the dry period. The bacteria distribution in the rainy period was notably absent of Cyanobacteria. Microcystis was observed in the dry period in which the gene cluster for cyanotoxins was found. Iron acquisition gene group was higher in the sample from the rainy season. This work mapped the first inventory of the planktonic microbial community of a large water reservoir in the Amazon, providing a reference for future functional studies and determining other communities and how they interact.

Original languageEnglish
Article numbere00523
JournalMicrobiologyOpen
Volume7
Issue number2
DOIs
StatePublished - 1 Apr 2018

Fingerprint

Cyanobacteria
Fresh Water
Microcystis
Betaproteobacteria
Plankton
Alphaproteobacteria
Water
Actinobacteria
Water Quality
Firearms
Multigene Family
Iron
Bacteria
Equipment and Supplies
Genes

Keywords

  • archaea
  • betaproteobacteria
  • cyanobacteria
  • massively parallel sequencing
  • microbial mapping
  • phage

Cite this

Cabral, B. C. A., Hoffmann, L., Budowle, B., Ürményi, T. P., Moura-Neto, R. S., Azevedo, S. M. F. O., & Silva, R. (2018). Planktonic microbial profiling in water samples from a Brazilian Amazonian reservoir. MicrobiologyOpen, 7(2), [e00523]. https://doi.org/10.1002/mbo3.523
Cabral, Bianca C.A. ; Hoffmann, Luísa ; Budowle, Bruce ; Ürményi, Turán P. ; Moura-Neto, Rodrigo S. ; Azevedo, Sandra M.F.O. ; Silva, Rosane. / Planktonic microbial profiling in water samples from a Brazilian Amazonian reservoir. In: MicrobiologyOpen. 2018 ; Vol. 7, No. 2.
@article{1b606ae77cf2485da2d5bad8462f1daf,
title = "Planktonic microbial profiling in water samples from a Brazilian Amazonian reservoir",
abstract = "Our comprehension of the dynamics and diversity of freshwater planktonic bacterial communities is far from complete concerning the Brazilian Amazonian region. Therefore, reference studies are urgently needed. We mapped bacterial communities present in the planktonic communities of a freshwater artificial reservoir located in the western Amazonian basin. Two samples were obtained from rainy and dry seasons, the periods during which water quality and plankton diversity undergo the most significant changes. Hypervariable 16S rRNA and shotgun sequencing were performed to describe the first reference of a microbial community in an Amazonian lentic system. Microbial composition consisted mainly of Betaproteobacteria, Cyanobacteria, Alphaproteobacteria, and Actinobacteria in the dry period. The bacteria distribution in the rainy period was notably absent of Cyanobacteria. Microcystis was observed in the dry period in which the gene cluster for cyanotoxins was found. Iron acquisition gene group was higher in the sample from the rainy season. This work mapped the first inventory of the planktonic microbial community of a large water reservoir in the Amazon, providing a reference for future functional studies and determining other communities and how they interact.",
keywords = "archaea, betaproteobacteria, cyanobacteria, massively parallel sequencing, microbial mapping, phage",
author = "Cabral, {Bianca C.A.} and Lu{\'i}sa Hoffmann and Bruce Budowle and {\"U}rm{\'e}nyi, {Tur{\'a}n P.} and Moura-Neto, {Rodrigo S.} and Azevedo, {Sandra M.F.O.} and Rosane Silva",
year = "2018",
month = "4",
day = "1",
doi = "10.1002/mbo3.523",
language = "English",
volume = "7",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

Cabral, BCA, Hoffmann, L, Budowle, B, Ürményi, TP, Moura-Neto, RS, Azevedo, SMFO & Silva, R 2018, 'Planktonic microbial profiling in water samples from a Brazilian Amazonian reservoir', MicrobiologyOpen, vol. 7, no. 2, e00523. https://doi.org/10.1002/mbo3.523

Planktonic microbial profiling in water samples from a Brazilian Amazonian reservoir. / Cabral, Bianca C.A.; Hoffmann, Luísa; Budowle, Bruce; Ürményi, Turán P.; Moura-Neto, Rodrigo S.; Azevedo, Sandra M.F.O.; Silva, Rosane.

In: MicrobiologyOpen, Vol. 7, No. 2, e00523, 01.04.2018.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Planktonic microbial profiling in water samples from a Brazilian Amazonian reservoir

AU - Cabral, Bianca C.A.

AU - Hoffmann, Luísa

AU - Budowle, Bruce

AU - Ürményi, Turán P.

AU - Moura-Neto, Rodrigo S.

AU - Azevedo, Sandra M.F.O.

AU - Silva, Rosane

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Our comprehension of the dynamics and diversity of freshwater planktonic bacterial communities is far from complete concerning the Brazilian Amazonian region. Therefore, reference studies are urgently needed. We mapped bacterial communities present in the planktonic communities of a freshwater artificial reservoir located in the western Amazonian basin. Two samples were obtained from rainy and dry seasons, the periods during which water quality and plankton diversity undergo the most significant changes. Hypervariable 16S rRNA and shotgun sequencing were performed to describe the first reference of a microbial community in an Amazonian lentic system. Microbial composition consisted mainly of Betaproteobacteria, Cyanobacteria, Alphaproteobacteria, and Actinobacteria in the dry period. The bacteria distribution in the rainy period was notably absent of Cyanobacteria. Microcystis was observed in the dry period in which the gene cluster for cyanotoxins was found. Iron acquisition gene group was higher in the sample from the rainy season. This work mapped the first inventory of the planktonic microbial community of a large water reservoir in the Amazon, providing a reference for future functional studies and determining other communities and how they interact.

AB - Our comprehension of the dynamics and diversity of freshwater planktonic bacterial communities is far from complete concerning the Brazilian Amazonian region. Therefore, reference studies are urgently needed. We mapped bacterial communities present in the planktonic communities of a freshwater artificial reservoir located in the western Amazonian basin. Two samples were obtained from rainy and dry seasons, the periods during which water quality and plankton diversity undergo the most significant changes. Hypervariable 16S rRNA and shotgun sequencing were performed to describe the first reference of a microbial community in an Amazonian lentic system. Microbial composition consisted mainly of Betaproteobacteria, Cyanobacteria, Alphaproteobacteria, and Actinobacteria in the dry period. The bacteria distribution in the rainy period was notably absent of Cyanobacteria. Microcystis was observed in the dry period in which the gene cluster for cyanotoxins was found. Iron acquisition gene group was higher in the sample from the rainy season. This work mapped the first inventory of the planktonic microbial community of a large water reservoir in the Amazon, providing a reference for future functional studies and determining other communities and how they interact.

KW - archaea

KW - betaproteobacteria

KW - cyanobacteria

KW - massively parallel sequencing

KW - microbial mapping

KW - phage

UR - http://www.scopus.com/inward/record.url?scp=85041137658&partnerID=8YFLogxK

U2 - 10.1002/mbo3.523

DO - 10.1002/mbo3.523

M3 - Article

VL - 7

JO - MicrobiologyOpen

JF - MicrobiologyOpen

SN - 2045-8827

IS - 2

M1 - e00523

ER -

Cabral BCA, Hoffmann L, Budowle B, Ürményi TP, Moura-Neto RS, Azevedo SMFO et al. Planktonic microbial profiling in water samples from a Brazilian Amazonian reservoir. MicrobiologyOpen. 2018 Apr 1;7(2). e00523. https://doi.org/10.1002/mbo3.523