Picosecond resolution of oxytocin tyrosyl fluorescence by 2 GHz frequency-domain fluorometry

Joseph R. Lakowicz; Gabor Laczko; Ignacy Gryczynski

doi:10.1016/0301-4622(86)80002-3

Picosecond resolution of oxytocin tyrosyl fluorescence by 2 GHz frequency-domain fluorometry

Joseph R. Lakowicz, Gabor Laczko, Ignacy Gryczynski

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The technique of frequency-domain fluorometry has been extended to 2000 MHz using the harmonic content of a picosecond laser source and a microchannel plate photomultiplier tube. This new instrument was used to resolve complex subnanosecond intensity and anisotropy decays of the tyrosyl emission of oxytocin. The intensity decay was found to contain at least three exponential components, 80, 359 and 927 ps. The anisotropy analysis revealed a 29 ps torsional motion of the tyrosine residue as well as a 454 ps overall rotational correlation time. The time resolution of this method should permit the comparison of experimental results with theoretical models for motions of proteins.

Original language	English
Pages (from-to)	97-100
Number of pages	4
Journal	Biophysical Chemistry
Volume	24
Issue number	2
DOIs	https://doi.org/10.1016/0301-4622(86)80002-3
State	Published - Jul 1986

Keywords

Anisotropy
Fluorescence spectroscopy
Frequency-domain fluorometry
Molecular dynamics
Oxytocin
Tyrosine fluorescence

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10.1016/0301-4622(86)80002-3

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@article{3686ac8265be48d9862add18c1b8562f,

title = "Picosecond resolution of oxytocin tyrosyl fluorescence by 2 GHz frequency-domain fluorometry",

abstract = "The technique of frequency-domain fluorometry has been extended to 2000 MHz using the harmonic content of a picosecond laser source and a microchannel plate photomultiplier tube. This new instrument was used to resolve complex subnanosecond intensity and anisotropy decays of the tyrosyl emission of oxytocin. The intensity decay was found to contain at least three exponential components, 80, 359 and 927 ps. The anisotropy analysis revealed a 29 ps torsional motion of the tyrosine residue as well as a 454 ps overall rotational correlation time. The time resolution of this method should permit the comparison of experimental results with theoretical models for motions of proteins.",

keywords = "Anisotropy, Fluorescence spectroscopy, Frequency-domain fluorometry, Molecular dynamics, Oxytocin, Tyrosine fluorescence",

author = "Lakowicz, {Joseph R.} and Gabor Laczko and Ignacy Gryczynski",

note = "Funding Information: This work was supported by grants PCM-8210878 and DMB-08502835 from the National Science Foundation and GM-29318 from the National Institutes of Health. J.R.L. offers his special thanks to Dr. Shinoda (Hatnmamatsu) for donating the microchannel plate photomultiplier tube. Additionally, J.R.L. thanks Mr. Fred Gonzalez (Coherent, Inc.) for his assistance in installing and aligning the picosecond laser system.",

year = "1986",

month = jul,

doi = "10.1016/0301-4622(86)80002-3",

language = "English",

volume = "24",

pages = "97--100",

journal = "Biophysical Chemistry",

issn = "0301-4622",

publisher = "Elsevier",

number = "2",

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TY - JOUR

T1 - Picosecond resolution of oxytocin tyrosyl fluorescence by 2 GHz frequency-domain fluorometry

AU - Lakowicz, Joseph R.

AU - Laczko, Gabor

AU - Gryczynski, Ignacy

N1 - Funding Information: This work was supported by grants PCM-8210878 and DMB-08502835 from the National Science Foundation and GM-29318 from the National Institutes of Health. J.R.L. offers his special thanks to Dr. Shinoda (Hatnmamatsu) for donating the microchannel plate photomultiplier tube. Additionally, J.R.L. thanks Mr. Fred Gonzalez (Coherent, Inc.) for his assistance in installing and aligning the picosecond laser system.

PY - 1986/7

Y1 - 1986/7

N2 - The technique of frequency-domain fluorometry has been extended to 2000 MHz using the harmonic content of a picosecond laser source and a microchannel plate photomultiplier tube. This new instrument was used to resolve complex subnanosecond intensity and anisotropy decays of the tyrosyl emission of oxytocin. The intensity decay was found to contain at least three exponential components, 80, 359 and 927 ps. The anisotropy analysis revealed a 29 ps torsional motion of the tyrosine residue as well as a 454 ps overall rotational correlation time. The time resolution of this method should permit the comparison of experimental results with theoretical models for motions of proteins.

AB - The technique of frequency-domain fluorometry has been extended to 2000 MHz using the harmonic content of a picosecond laser source and a microchannel plate photomultiplier tube. This new instrument was used to resolve complex subnanosecond intensity and anisotropy decays of the tyrosyl emission of oxytocin. The intensity decay was found to contain at least three exponential components, 80, 359 and 927 ps. The anisotropy analysis revealed a 29 ps torsional motion of the tyrosine residue as well as a 454 ps overall rotational correlation time. The time resolution of this method should permit the comparison of experimental results with theoretical models for motions of proteins.

KW - Anisotropy

KW - Fluorescence spectroscopy

KW - Frequency-domain fluorometry

KW - Molecular dynamics

KW - Oxytocin

KW - Tyrosine fluorescence

UR - http://www.scopus.com/inward/record.url?scp=0022538756&partnerID=8YFLogxK

U2 - 10.1016/0301-4622(86)80002-3

DO - 10.1016/0301-4622(86)80002-3

M3 - Article

C2 - 3756310

AN - SCOPUS:0022538756

SN - 0301-4622

VL - 24

SP - 97

EP - 100

JO - Biophysical Chemistry

JF - Biophysical Chemistry

IS - 2

ER -

Picosecond resolution of oxytocin tyrosyl fluorescence by 2 GHz frequency-domain fluorometry

Abstract

Keywords

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