Photostability of Cy3 and Cy5-Labeled DNA in the Presence of Metallic Silver Particles

Joanna Malicka, Ignacy Gryczynski, Jiyu Fang, Jozef Kusba, Joseph R. Lakowicz

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

We examined the photostability of a double-stranded DNA oligomer, covalently labeled with Cy3 or Cy5 on one strand, in the presence of metallic silver island films. In our experimental configuration a minor fraction of the labeled DNA was close to the silver particles and the remainder was distant from the panicles. Proximity of the fluorophores to silver island films resulted in increased intensity. Upon continuous illumination we found a fraction of the emission that was resistant to the photobleaching. The emission spectra of the residual fractions were identical to the initial spectra. The frequency-domain lifetime measurements of this fraction revealed greatly shortened decay times. These results are consistent with the photostable fraction being close to the silver particles. This results suggest that the number of photons detected per fluorophore, prior to photobleaching, can be increased 5-fold or more by proximity to silver particles. Localization at an optimal distance from the silver surface may result in larger enhancements.

Original languageEnglish
Pages (from-to)439-447
Number of pages9
JournalJournal of Fluorescence
Volume12
Issue number3-4
DOIs
StatePublished - Dec 2002

Keywords

  • Colloids
  • Cy3
  • Cy5
  • DNA
  • Metal-enhanced fluorescence
  • Photostability
  • Silver islands

Fingerprint Dive into the research topics of 'Photostability of Cy3 and Cy5-Labeled DNA in the Presence of Metallic Silver Particles'. Together they form a unique fingerprint.

  • Cite this