Photoselection of luminescent molecules in anisotropic media in the case of two-photon excitation. Part II. Experimental studies of Hoechst 33342 in stretched poly(vinyl alcohol) films

Alfons Kawski, Z. Gryczynski, I. Gryczyński, J. R. Lakowicz, G. Piszczek

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

It was found by investigating dichroism and emission anisotropy in the case of one- and two-photon excitation of Hoechst 33342 [bis-benzimide,2,5′-bi-lH-benzimidazole, 2'-(4-ethoxyphenyl)-5-5(4-methyl-1-piperazinyl)] in stretched poly(vinyl alcohol) (PVA) films, that the absorption and fluorescence transition moments lie along the long molecular axis of the molecule studied. The slight deviation of the transition moment direction in fluorescence (about 8°) from that in absorption can be due to the incomplete linearity of the Hoechst molecule.

Original languageEnglish
Pages (from-to)1037-1041
Number of pages5
JournalZeitschrift fur Naturforschung - Section A Journal of Physical Sciences
Volume51
Issue number9
DOIs
StatePublished - Sep 1996

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