It was found by investigating dichroism and emission anisotropy in the case of one- and two-photon excitation of Hoechst 33342 [bis-benzimide,2,5′-bi-lH-benzimidazole, 2'-(4-ethoxyphenyl)-5-5(4-methyl-1-piperazinyl)] in stretched poly(vinyl alcohol) (PVA) films, that the absorption and fluorescence transition moments lie along the long molecular axis of the molecule studied. The slight deviation of the transition moment direction in fluorescence (about 8°) from that in absorption can be due to the incomplete linearity of the Hoechst molecule.
|Number of pages||5|
|Journal||Zeitschrift fur Naturforschung - Section A Journal of Physical Sciences|
|State||Published - Sep 1996|