Photoprotective role of the xanthophyll cycle studied by means of modeling of xanthophyll-LHCII interactions

Wieslaw I. Gruszecki; Monika Zubik; Rafal Luchowski; Ewa Janik; Wojciech Grudzinski; Malgorzata Gospodarek; Jacek Goc; Leszek Fiedor; Zygmunt Gryczynski; Ignacy Gryczynski

doi:10.1016/j.chemphys.2010.03.025

Photoprotective role of the xanthophyll cycle studied by means of modeling of xanthophyll-LHCII interactions

Wieslaw I. Gruszecki, Monika Zubik, Rafal Luchowski, Ewa Janik, Wojciech Grudzinski, Malgorzata Gospodarek, Jacek Goc, Leszek Fiedor, Zygmunt Gryczynski, Ignacy Gryczynski

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13 Scopus citations

Abstract

The problem of photoprotection associated with the xanthophyll cycle is addressed by examination of effects of exogenous violaxanthin and zeaxanthin on isolated antenna complex LHCII. Analysis of electronic absorption spectra suggests multiple sites of binding of violaxanthin and zeaxanthin to the protein environment. Xanthophyll binding results in enhancement of excitonic bands at ca. 530 and 740 nm, as concluded on the basis of Resonance Light Scattering spectra. The energy states attributed to these excitonic bands are concluded to quench singlet excitations. Fluorescence Lifetime Imaging Microscopy shows that both xanthophylls, but in particular zeaxanthin, promote formation of supramolecular structures characterized by extremely low fluorescence yield. Photoprotective meaning of the xanthophyll cycle is concluded to be primarily based upon role of zeaxanthin in promoting formation of supramolecular structures of LHCII characterized by high rate of energy dissipation.

Original language	English
Pages (from-to)	122-128
Number of pages	7
Journal	Chemical Physics
Volume	373
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chemphys.2010.03.025
State	Published - 19 Jul 2010

Keywords

Carotenoids
LHCII
Photoprotection
Photosynthesis
Xanthophyll cycle

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@article{5c87cba896be4a0ba4b8767dd0fb6dd3,

title = "Photoprotective role of the xanthophyll cycle studied by means of modeling of xanthophyll-LHCII interactions",

abstract = "The problem of photoprotection associated with the xanthophyll cycle is addressed by examination of effects of exogenous violaxanthin and zeaxanthin on isolated antenna complex LHCII. Analysis of electronic absorption spectra suggests multiple sites of binding of violaxanthin and zeaxanthin to the protein environment. Xanthophyll binding results in enhancement of excitonic bands at ca. 530 and 740 nm, as concluded on the basis of Resonance Light Scattering spectra. The energy states attributed to these excitonic bands are concluded to quench singlet excitations. Fluorescence Lifetime Imaging Microscopy shows that both xanthophylls, but in particular zeaxanthin, promote formation of supramolecular structures characterized by extremely low fluorescence yield. Photoprotective meaning of the xanthophyll cycle is concluded to be primarily based upon role of zeaxanthin in promoting formation of supramolecular structures of LHCII characterized by high rate of energy dissipation.",

keywords = "Carotenoids, LHCII, Photoprotection, Photosynthesis, Xanthophyll cycle",

author = "Gruszecki, {Wieslaw I.} and Monika Zubik and Rafal Luchowski and Ewa Janik and Wojciech Grudzinski and Malgorzata Gospodarek and Jacek Goc and Leszek Fiedor and Zygmunt Gryczynski and Ignacy Gryczynski",

note = "Funding Information: This research was financed by the Ministry of Science and Higher Education of Poland from the funds for science in the years 2008–2011 within the research project N N303 285034 . RL acknowledges the post-doctoral fellowship from the Ministry of Science and Higher Education of Poland (Grant No. 17/MOB/2007/0 ).",

year = "2010",

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day = "19",

doi = "10.1016/j.chemphys.2010.03.025",

language = "English",

volume = "373",

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journal = "Chemical Physics",

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T1 - Photoprotective role of the xanthophyll cycle studied by means of modeling of xanthophyll-LHCII interactions

AU - Gruszecki, Wieslaw I.

AU - Zubik, Monika

AU - Luchowski, Rafal

AU - Janik, Ewa

AU - Grudzinski, Wojciech

AU - Gospodarek, Malgorzata

AU - Goc, Jacek

AU - Fiedor, Leszek

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

N1 - Funding Information: This research was financed by the Ministry of Science and Higher Education of Poland from the funds for science in the years 2008–2011 within the research project N N303 285034 . RL acknowledges the post-doctoral fellowship from the Ministry of Science and Higher Education of Poland (Grant No. 17/MOB/2007/0 ).

PY - 2010/7/19

Y1 - 2010/7/19

N2 - The problem of photoprotection associated with the xanthophyll cycle is addressed by examination of effects of exogenous violaxanthin and zeaxanthin on isolated antenna complex LHCII. Analysis of electronic absorption spectra suggests multiple sites of binding of violaxanthin and zeaxanthin to the protein environment. Xanthophyll binding results in enhancement of excitonic bands at ca. 530 and 740 nm, as concluded on the basis of Resonance Light Scattering spectra. The energy states attributed to these excitonic bands are concluded to quench singlet excitations. Fluorescence Lifetime Imaging Microscopy shows that both xanthophylls, but in particular zeaxanthin, promote formation of supramolecular structures characterized by extremely low fluorescence yield. Photoprotective meaning of the xanthophyll cycle is concluded to be primarily based upon role of zeaxanthin in promoting formation of supramolecular structures of LHCII characterized by high rate of energy dissipation.

AB - The problem of photoprotection associated with the xanthophyll cycle is addressed by examination of effects of exogenous violaxanthin and zeaxanthin on isolated antenna complex LHCII. Analysis of electronic absorption spectra suggests multiple sites of binding of violaxanthin and zeaxanthin to the protein environment. Xanthophyll binding results in enhancement of excitonic bands at ca. 530 and 740 nm, as concluded on the basis of Resonance Light Scattering spectra. The energy states attributed to these excitonic bands are concluded to quench singlet excitations. Fluorescence Lifetime Imaging Microscopy shows that both xanthophylls, but in particular zeaxanthin, promote formation of supramolecular structures characterized by extremely low fluorescence yield. Photoprotective meaning of the xanthophyll cycle is concluded to be primarily based upon role of zeaxanthin in promoting formation of supramolecular structures of LHCII characterized by high rate of energy dissipation.

KW - Carotenoids

KW - LHCII

KW - Photoprotection

KW - Photosynthesis

KW - Xanthophyll cycle

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SN - 0301-0104

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EP - 128

JO - Chemical Physics

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ER -

Photoprotective role of the xanthophyll cycle studied by means of modeling of xanthophyll-LHCII interactions

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