Photophysical properties of Cerulean and Venus fluorescent proteins

Pabak Sarkar, Srinagesh V. Koushik, Steven S. Vogel, Ignacy Gryczynski, Zygmunt Gryczynski

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Cerulean and Venus are recently developed fluorescent proteins, often used as a donor-acceptor pair by researchers in Förster resonance energy transfer-based colocalization studies. We characterized the fluorescent properties of these two proteins in a broad spectral range (form ultraviolet to visible region). Excitation spectra, lifetimes, and polarization spectra show significant energy transfer from aromatic amino acids to the fluorescent protein chromophore. High steady-state anisotropy values and the lack of a fast component in anisotropy decays show that the fluorescent protein chromophore is rigidly fixed within the protein structure. Furthermore, we show that the chromophores are not accessible to external quenchers, such as acrylamide or potassium iodide (KI), allowing the removal of "unwanted" background in the environment with external quencher, while leaving the Cerulean/Venus fluorescence unchanged.

Original languageEnglish
Article number034047
JournalJournal of Biomedical Optics
Volume14
Issue number3
DOIs
StatePublished - 1 Dec 2009

Fingerprint

Venus (planet)
proteins
Proteins
Chromophores
chromophores
Energy transfer
Anisotropy
energy transfer
Potassium iodide
potassium iodides
Potassium Iodide
Aromatic Amino Acids
anisotropy
Acrylamide
Carboxylic acids
iodides
amino acids
Amino acids
Fluorescence
Polarization

Keywords

  • Förster resonance energy transfer
  • fluorescence anisotropy
  • fluorescence timeline

Cite this

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abstract = "Cerulean and Venus are recently developed fluorescent proteins, often used as a donor-acceptor pair by researchers in F{\"o}rster resonance energy transfer-based colocalization studies. We characterized the fluorescent properties of these two proteins in a broad spectral range (form ultraviolet to visible region). Excitation spectra, lifetimes, and polarization spectra show significant energy transfer from aromatic amino acids to the fluorescent protein chromophore. High steady-state anisotropy values and the lack of a fast component in anisotropy decays show that the fluorescent protein chromophore is rigidly fixed within the protein structure. Furthermore, we show that the chromophores are not accessible to external quenchers, such as acrylamide or potassium iodide (KI), allowing the removal of {"}unwanted{"} background in the environment with external quencher, while leaving the Cerulean/Venus fluorescence unchanged.",
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Photophysical properties of Cerulean and Venus fluorescent proteins. / Sarkar, Pabak; Koushik, Srinagesh V.; Vogel, Steven S.; Gryczynski, Ignacy; Gryczynski, Zygmunt.

In: Journal of Biomedical Optics, Vol. 14, No. 3, 034047, 01.12.2009.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Photophysical properties of Cerulean and Venus fluorescent proteins

AU - Sarkar, Pabak

AU - Koushik, Srinagesh V.

AU - Vogel, Steven S.

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

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AB - Cerulean and Venus are recently developed fluorescent proteins, often used as a donor-acceptor pair by researchers in Förster resonance energy transfer-based colocalization studies. We characterized the fluorescent properties of these two proteins in a broad spectral range (form ultraviolet to visible region). Excitation spectra, lifetimes, and polarization spectra show significant energy transfer from aromatic amino acids to the fluorescent protein chromophore. High steady-state anisotropy values and the lack of a fast component in anisotropy decays show that the fluorescent protein chromophore is rigidly fixed within the protein structure. Furthermore, we show that the chromophores are not accessible to external quenchers, such as acrylamide or potassium iodide (KI), allowing the removal of "unwanted" background in the environment with external quencher, while leaving the Cerulean/Venus fluorescence unchanged.

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