Photophysical properties of a new DyLight 594 dye

Pabak Sarkar; Savitha Sridharan; Rafal Luchowski; Surbhi Desai; Boguslawa Dworecki; Marie Nlend; Zygmunt Gryczynski; Ignacy Gryczynski

doi:10.1016/j.jphotobiol.2009.10.005

Photophysical properties of a new DyLight 594 dye

Pabak Sarkar, Savitha Sridharan, Rafal Luchowski, Surbhi Desai, Boguslawa Dworecki, Marie Nlend, Zygmunt Gryczynski, Ignacy Gryczynski

Microbiology, Immunology & Genetics

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We describe spectral properties of novel fluorescence probe DyLight™ 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50%. The new DyLight dye also shows slightly longer lifetime and photostability. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make this fluorophore attractive as a fluorescence probe in biochemical/biological studies involving fluorescence methods.

Original language	English
Pages (from-to)	35-39
Number of pages	5
Journal	Journal of Photochemistry and Photobiology B: Biology
Volume	98
Issue number	1
DOIs	https://doi.org/10.1016/j.jphotobiol.2009.10.005
State	Published - 21 Jan 2010

Keywords

Anisotropy
Fluorescence lifetime
Fluorophore
Quantum yield

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10.1016/j.jphotobiol.2009.10.005

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title = "Photophysical properties of a new DyLight 594 dye",

abstract = "We describe spectral properties of novel fluorescence probe DyLight{\texttrademark} 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50%. The new DyLight dye also shows slightly longer lifetime and photostability. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make this fluorophore attractive as a fluorescence probe in biochemical/biological studies involving fluorescence methods.",

keywords = "Anisotropy, Fluorescence lifetime, Fluorophore, Quantum yield",

author = "Pabak Sarkar and Savitha Sridharan and Rafal Luchowski and Surbhi Desai and Boguslawa Dworecki and Marie Nlend and Zygmunt Gryczynski and Ignacy Gryczynski",

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AU - Sarkar, Pabak

AU - Sridharan, Savitha

AU - Luchowski, Rafal

AU - Desai, Surbhi

AU - Dworecki, Boguslawa

AU - Nlend, Marie

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

PY - 2010/1/21

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AB - We describe spectral properties of novel fluorescence probe DyLight™ 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50%. The new DyLight dye also shows slightly longer lifetime and photostability. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make this fluorophore attractive as a fluorescence probe in biochemical/biological studies involving fluorescence methods.

KW - Anisotropy

KW - Fluorescence lifetime

KW - Fluorophore

KW - Quantum yield

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JO - Journal of Photochemistry and Photobiology, B: Biology

JF - Journal of Photochemistry and Photobiology, B: Biology

IS - 1

ER -

Photophysical properties of a new DyLight 594 dye

Abstract

Keywords

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