Photophysical properties of a new DyLight 594 dye

Pabak Sarkar, Savitha Sridharan, Rafal Luchowski, Surbhi Desai, Boguslawa Dworecki, Marie Nlend, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We describe spectral properties of novel fluorescence probe DyLight™ 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50%. The new DyLight dye also shows slightly longer lifetime and photostability. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make this fluorophore attractive as a fluorescence probe in biochemical/biological studies involving fluorescence methods.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalJournal of Photochemistry and Photobiology B: Biology
Volume98
Issue number1
DOIs
StatePublished - 21 Jan 2010

Keywords

  • Anisotropy
  • Fluorescence lifetime
  • Fluorophore
  • Quantum yield

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