Abstract
We describe spectral properties of novel fluorescence probe DyLight™ 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50%. The new DyLight dye also shows slightly longer lifetime and photostability. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make this fluorophore attractive as a fluorescence probe in biochemical/biological studies involving fluorescence methods.
Original language | English |
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Pages (from-to) | 35-39 |
Number of pages | 5 |
Journal | Journal of Photochemistry and Photobiology B: Biology |
Volume | 98 |
Issue number | 1 |
DOIs | |
State | Published - 21 Jan 2010 |
Keywords
- Anisotropy
- Fluorescence lifetime
- Fluorophore
- Quantum yield